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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C15672-100 100 µl $530 
Antibody

Monoclonal Mouse anti‑Human HSPB1 / HSP27 Antibody (IHC, IF, WB) LS‑C15672

Monoclonal Mouse anti‑Human HSPB1 / HSP27 Antibody (IHC, IF, WB) LS‑C15672

Antibody:
Mouse Monoclonal to Human HSPB1 / HSP27
Application:
IHC, IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$530 
LS-C15672-100
100 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human HSPB1 / HSP27
Application:
IHC, IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
HSP27 antibody LS-C15672 is an unconjugated mouse monoclonal antibody to HSP27 (HSPB1) from human. It is reactive with human and monkey. Validated for ELISA, ICC, IF, IHC and WB.
Target
Human HSPB1 / HSP27
Synonyms
HSPB1 | 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
Host
Mouse
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Immunofluorescence
  • Western blot (1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length HSP27 protein. MW of Antigen: 27kD.
Specificity
Recognizes endogenous levels of total HSP27 protein. Does not cross-react with other heat shock proteins. Species cross-reactivity: Human and monkey.
Usage
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50. Immunocytochemistry (for IF): 1:200.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPB1 / HSP27
P04792 NM_001540 NP_001531.1

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Requested From: United States
Date Requested: 7/4/2020