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| Catalog Number | Size | Price |
|---|---|---|
| LS-C15718-50 | 50 µg | $611 |
| LS-C15718-200 | 200 µg | $1,029 |
Monoclonal Mouse anti‑Human HSPB1 / HSP27 Antibody (IHC, IF, WB) LS‑C15718
Monoclonal Mouse anti‑Human HSPB1 / HSP27 Antibody (IHC, IF, WB) LS‑C15718
Antibody:
HSPB1 / HSP27 Mouse anti-Human Monoclonal Antibody
Application:
IHC, ICC, IF, WB, IP, ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
HSPB1 / HSP27 Mouse anti-Human Monoclonal Antibody
Application:
IHC, ICC, IF, WB, IP, ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Specifications
Description
HSP27 antibody LS-C15718 is an unconjugated mouse monoclonal antibody to HSP27 (HSPB1) from human. It is reactive with human and monkey. Validated for ELISA, ICC, IF, IHC, IP and WB.
Target
Human HSPB1 / HSP27
Synonyms
HSPB1 | 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
Host
Mouse
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Purified Hsp24 isolated from MCF-7, a human breast tumor cell line (7). Upon cloning and sequence analysis of the gene encoding the 24kD protein, nucleotide sequence comparison revealed complete identity with the small heat shock protein, Hsp27.
Specificity
Recognizes an ~27kD protein, corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots. Species cross-reactivity: Human, monkey and fish (rainbow trout). It does not exhibit reactivity with rodent Hsp25, alphaA, alphaB or beta-crystallin proteins.
Applications
- IHC (5 µg/ml)
- ICC (10 µg/ml)
- Immunofluorescence
- Western blot
- Immunoprecipitation (12.5 µg/ml)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in ELISA, Western Blot and Immunofluorescence. Western: Blot (Colorimetric): 1 ug/mL. Immunoprecipitation: 12.5 ug/mL. Immunohistochemistry: 5 ug/mL. Immunocytochemistry: 10 ug/mL. Immunoelectron Microscopy: 1:50. Positive control: HeLa Heat Shocked Cell Lysate and Recombinant Human Hsp27 Protein.
Presentation
PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HSPB1 / HSP27
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Species:
Turkey, Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
Species:
Human, Chimpanzee, Mouse, Rat, Sheep, Chicken
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Human, Monkey, Bovine, Guinea pig, Pig
Applications:
Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
