Rat HSPA8 / HSC70

Mouse

Rat (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated

Protein G purified

Unmodified

A preparation of spinach Hsc70 proteins comprised of cytosolic Hsc70, BiP and possibly other members of the Hsc70 family of proteins.

This affinity purified antibody Detects a ~78kD protein, corresponding to the apparent molecular mass of Hsc70 plant BiP on SDS-PAGE immunoblots, in samples from apple, asparagus, carrot, corn, lettuce, orange, potato, spinach, tomato, Arabidopsis thaliana leaves, seeds and siliques, woody plant bark tissue and tobacco. Although this antibody can be used for immunoprecipitations under native conditions, it is not recommended for immunoprecipitating BiP from tomato lysates. This antibody does not cross react with E. coli DnaK, mammalian Hsc70, recombinant hamster BiP or spinach cytosolic Hsc70.

• Western blot
• Immunoprecipitation (10 µg/ml)

Western Blot (Colorimetric): 1 ug/ml. Western Blot (ECL). Immunoprecipitation: 10 ug/ml.

PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P11142 NCBI: NM_006597 NP_006588.1