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Catalog Number Size Price
LS-C777172-100 100 µg (1 mg/ml) $472 
HSPA5 / GRP78 / BiP Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.
HSPA5 / GRP78 / BiP Antibody - Western Blot analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.
HSPA5 / GRP78 / BiP Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.
HSPA5 / GRP78 / BiP Antibody - Western Blot analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.
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Monoclonal Mouse anti‑Human HSPA5 / GRP78 / BiP Antibody (clone 3C5‑1A4, Atto 488, IF, WB) LS‑C777172

Monoclonal Mouse anti‑Human HSPA5 / GRP78 / BiP Antibody (clone 3C5‑1A4, Atto 488, IF, WB) LS‑C777172

Antibody:
HSPA5 / GRP78 / BiP Mouse anti-Human Monoclonal (Atto 488) (3C5-1A4) Antibody
Application:
IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Atto 488, Unmodified
Other formats:
Price
Catalog Number
$472
LS-C777172-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HSPA5 / GRP78 / BiP Mouse anti-Human Monoclonal (Atto 488) (3C5-1A4) Antibody
Application:
IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Atto 488, Unmodified
Other formats:

Specifications

Description
BiP antibody LS-C777172 is an Atto 488-conjugated mouse monoclonal antibody to BiP (HSPA5 / GRP78) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF and WB.
Target
Human HSPA5 / GRP78 / BiP
Synonyms
HSPA5 | BIP | Heat shock 70 kDa protein 5 | MIF2 | GRP-78 | GRP78
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
3C5-1A4
Conjugations
Atto 488. Also available conjugated with AP, APC, Atto 390, Atto 594, Biotin, FITC, PerCP, RPE.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Full-length recombinant rat GRP78.
Specificity
Detects ~78 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • ELISA
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPA5 / GRP78 / BiP
P11021 M19645 AAA52614.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024