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Catalog Number Size Price
LS-C745306-25 25 µl (0.96 mg/ml) $275 
HSP90AA1 / Hsp90 Alpha A1 Antibody - affinity purified anti-Hsp90 acetyl K294 antibody was used at 20 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and granular cytoplasmic positive staining in human prostate carcinoma at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
HSP90AA1 / Hsp90 Alpha A1 Antibody - Western blot using the Affinity Purified anti-Hsp90 acetyl K294 antibody shows detection of a band at ~90 kDa corresponding to Hsp90 in an SkBr3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor). Western blotting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA). Immunoprecipitation with anti-Hsp90 was performed prior to western blotting with anti-Hsp90 acetyl K294.
HSP90AA1 / Hsp90 Alpha A1 Antibody - affinity purified anti-Hsp90 acetyl K294 antibody was used at 20 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and granular cytoplasmic positive staining in human prostate carcinoma at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
HSP90AA1 / Hsp90 Alpha A1 Antibody - Western blot using the Affinity Purified anti-Hsp90 acetyl K294 antibody shows detection of a band at ~90 kDa corresponding to Hsp90 in an SkBr3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor). Western blotting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA). Immunoprecipitation with anti-Hsp90 was performed prior to western blotting with anti-Hsp90 acetyl K294.
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Polyclonal Rabbit anti‑Human HSP90AA1 / Hsp90 Alpha A1 Antibody (K294, IHC, WB) LS‑C745306

Polyclonal Rabbit anti‑Human HSP90AA1 / Hsp90 Alpha A1 Antibody (K294, IHC, WB) LS‑C745306

Antibody:
HSP90AA1 / Hsp90 Alpha A1 Rabbit anti-Human Polyclonal (K294) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745306-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
HSP90AA1 / Hsp90 Alpha A1 Rabbit anti-Human Polyclonal (K294) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Hsp90 Alpha A1 antibody LS-C745306 is an unconjugated rabbit polyclonal antibody to Hsp90 Alpha A1 (HSP90AA1) (K294) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Target
Human HSP90AA1 / Hsp90 Alpha A1
Synonyms
HSP90AA1 | EL52 | HSP86 | Hsp89 | HSP89A | HSPC1 | Heat shock 86 kDa | Hsp90 | HSP90A | HSP90N | HSPCA | HSPCAL4 | HSPN | LAP2 | HSP 86 | HSPCAL1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Monkey, Chicken, Drosophila (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Anti-Heat Shock Protein HSP 90-alpha acetyl specific K294 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding K294 of human Hsp90.
Epitope
K294
Specificity
Anti-Heat Shock Protein HSP 90-alpha acetyl specific K294 antibody is directed against human Hsp90 protein acetylated at K294.  While ELISA data show strong reactivity with the acetylated form of the immunizing peptide and minimal reactivity with the non-acetylated form, to date western blotting data are not definitive for acetyl K294 specificity as blots show equivalent staining of lysates from cells either treated or untreated with Trichostatin A (an HDAC inhibitor). A BLAST analysis was used to suggest cross-reactivity with Hsp90 from human, mouse, rat, monkey, chicken and Drosophila based on 100% homology with the immunizing sequence. Reactivity of this antibody with Hsp90 from other species is unknown.
Applications
  • IHC (5 - 10 µg/ml)
  • Western blot (1:500 - 1:2500)
  • ELISA (1:2500 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSP90AA1 / Hsp90 Alpha A1
P07900 NM_005348 NP_005339.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021