Human HSP90AA1 / Hsp90 Alpha A1

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Monkey, Chicken, Drosophila (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity chromatography

Unmodified

Anti-Heat Shock Protein HSP 90-alpha acetyl specific K294 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding K294 of human Hsp90.

K294

Anti-Heat Shock Protein HSP 90-alpha acetyl specific K294 antibody is directed against human Hsp90 protein acetylated at K294.  While ELISA data show strong reactivity with the acetylated form of the immunizing peptide and minimal reactivity with the non-acetylated form, to date western blotting data are not definitive for acetyl K294 specificity as blots show equivalent staining of lysates from cells either treated or untreated with Trichostatin A (an HDAC inhibitor). A BLAST analysis was used to suggest cross-reactivity with Hsp90 from human, mouse, rat, monkey, chicken and Drosophila based on 100% homology with the immunizing sequence. Reactivity of this antibody with Hsp90 from other species is unknown.

• IHC (5 - 10 µg/ml)
• Western blot (1:500 - 1:2500)
• ELISA (1:2500 - 1:10000)

Applications should be user optimized.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P07900 NCBI: NM_005348 NP_005339.3