Identifies purified bovine and human (alpha and beta) Hsp90 and detects a 90kD protein, corresponding to the apparent molecular mass of Hsp90 on SDS-PAGE immunoblots, in samples from human, mouse, rat, beluga, bovine, canine, C. elegans, chicken, fish (brook trout, carp, chinook salmon, chum salmon, medaka, rainbow trout), guinea pig, hamster, lobster, monkey, mussel, porcine, rabbit, scallop, sheep and water mold. Reacts with rodent glucocorticoid receptor, 95kD. Also reacts with proteins called proliferation potential proteins (P2PS), ~30-40kD. These proteins are associated with the 30-40S substructures of nuclear hnRNP complexes and they share an epitope in common with Hsp90. The epitope has been mapped to amino acids 604-697 of the human Hsp90 sequence. Detects both Hsp90 alpha and beta.
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry and FACS. Western Blot (Colorimetric): 1 ug/ml. Immunoprecipitation: 4 ug/ml. Does not appear to react well with Hsp90 that is bound to steroid receptors or to pp60v-src of Rouse sarcoma virus. Positive control: Hsp90 (Human) Protein and HeLa (Heat Shocked) Cell Lysate.
10 mM PBS, pH 7.2, 0.1 mM PMSF, 50% glycerol.
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
This antibody carries the LSBio 100% Guarantee.
About HSP90AA1 / Hsp90 Alpha A1