Target
Human HSP70 / Heat Shock Protein 70
Reactivity
Human, Monkey, Bovine
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
HSP70 protein from HeLa cells. Molecular Weight of Antigen: Inducible: 72kD, Constitutive: 73kD. Cellular Localization: Nuclear and cytoplasmic.
Specificity
Recognizes both the constitutive (HSP73) and inducible (HSP72) forms of HSP70. Species cross-reactivity: Human, monkey and bovine.
Applications
- IHC
- IHC - Paraffin
- Immunofluorescence
- Western blot (0.5 - 1 µg/ml)
- Immunoprecipitation
- Flow Cytometry
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native & denatured): 2 ug antibody/mg protein lysate using Protein A. Western Blot: 0.5-1 ug/ml for 2 hours at RT. Immunohistochemistry (Formalin/paraffin): 2-4 ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Positive control: BT474 or HeLa cells, breast carcinoma.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 40% Glycerol, 0.2% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee