Human HSP70 / Heat Shock Protein 70
Human, Monkey, Bovine
(tested or 100% immunogen sequence identity)
Protein A purified
HSP70 protein from HeLa cells. Molecular Weight of Antigen: Inducible: 72kD, Constitutive: 73kD. Cellular Localization: Nuclear and cytoplasmic.
Recognizes both the constitutive (HSP73) and inducible (HSP72) forms of HSP70. Species cross-reactivity: Human, monkey and bovine.
- IHC - Paraffin
- Western blot (0.5 - 1 µg/ml)
- Flow Cytometry
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Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native & denatured): 2 ug antibody/mg protein lysate using Protein A. Western Blot: 0.5-1 ug/ml for 2 hours at RT. Immunohistochemistry (Formalin/paraffin): 2-4 ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Positive control: BT474 or HeLa cells, breast carcinoma.
PBS, pH 7.4, 0.09% Sodium Azide, 40% Glycerol, 0.2% BSA
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.