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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C186515-100 100 µg (0.5 mg/ml) $405 
HSP31 / Hsp31p Antibody - HSP31 antibody (0.05 ug/ml) staining of yeast lysate (wt) and the KO (delta) in the left panel and the Ponceau stain in the right panel (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained by Dr. M. Skoneczny, IBB, Warsaw, Poland.

HSP31 / Hsp31p Antibody (aa50‑64) LS‑C186515

HSP31 / Hsp31p Antibody (aa50‑64) LS‑C186515

Antibody:
Goat Polyclonal to Yeast HSP31 / Hsp31p
Application:
WB, Peptide-ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified
Price
Catalog Number
$405 
LS-C186515-100
100 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Yeast HSP31 / Hsp31p
Application:
WB, Peptide-ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified

Specifications

Description
Hsp31p antibody LS-C186515 is an unconjugated goat polyclonal antibody to yeast Hsp31p (HSP31 ). Validated for Peptide-ELISA and WB.
Target
Yeast HSP31 / Hsp31p
Host
Goat
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • Western blot (0.02 - 0.06 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Immunogen
Peptide with sequence C-ETGKFGWDEHSLAKD, from the internal region of the protein sequence according to NP_010822.1.
Blocking Peptide
LS-E27900 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C186515. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa50-64
Specificity
Yeast HSP31 / Hsp31p
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 24kD band observed in lysates of postdiauxic (overnight grown yeast cells, and no signal in KO grown under identical conditions. Cells grown in logarithmic phase show diminished signals as expected. (calculated MW of 25.7kD according to NP_010822.1). Recommended concentration: 0.02-0.06 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSP31 / Hsp31p
NP_010822.1

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Requested From: United States
Date Requested: 10/14/2019