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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2682-50 | 50 µg (1 mg/ml) | $485 |
IHC‑plus™ Polyclonal Rabbit anti‑Human HSF1 Antibody (IHC, WB) LS‑B2682
IHC‑plus™ Polyclonal Rabbit anti‑Human HSF1 Antibody (IHC, WB) LS‑B2682
Note: This antibody replaces LS-C7378
Antibody:
HSF1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Rabbit
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
HSF1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Rabbit
Format:
Unconjugated, Unmodified
Specifications
Description
HSF1 antibody LS-B2682 is an unconjugated rabbit polyclonal antibody to HSF1 from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HSF1
Synonyms
HSF1 | HSTF 1 | Heat shock factor protein 1 | HSF 1 | HSTF1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant human HSF-1 expressed in E. coli
Specificity
Recognizes human HSF-1 at ~85kD protein in unstressed cells and an ~95kD protein in heat shocked cells, corresponding to the molecular masses of the inactive and activated forms respectively of HSF-1. Species cross-reactivity: mouse, rat, monkey, rabbit.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:500)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2682 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2682 was determined to be 5 ug/ml. Western Blot: Positive control: Recombinant Human HSF-1 Protein.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HSF1
Validation
Anti-HSF1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HSF1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ HSF1 Antibody for IHC, WB/Western LS-B2682 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Rabbit
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA, Gel shift
Species:
Human
Applications:
IHC, Western blot, Immunoprecipitation
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Reactivity:
Mouse
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
Date Requested: 4/17/2024
