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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HOXD8 Antibody (aa243‑270) LS‑C159867
HOXD8 antibody LS-C159867 is an unconjugated rabbit polyclonal antibody to human HOXD8. Validated for IF and WB.
Catalog
Size
Price
LS-C159867-400
400 µl
$305

Popular HOXD8 Products

Western Blot analysis of HOXD8 expression in transfected 293T cell line by HOXD8 monoclonal antibody (M01), clone 10F8.Lane 1: HOXD8 transfected lysate(31.8 KDa).Lane 2: Non-transfected lysate.
Species: Human
Applications: Western blot, ELISA, RNA interference
Antibody
Species: Human, Mouse, Rat
Applications: ICC, Western blot
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

HOXD8 antibody LS-C159867 is an unconjugated rabbit polyclonal antibody to human HOXD8. Validated for IF and WB.

Specifications

Target
Human HOXD8
Synonyms
HOXD8, Homeo box 4E, Homeobox D8, Homeobox protein 5.4, Homeobox protein Hox-4E, Homeobox protein Hox-D8, HOX4, HOX5.4, Homeo box D8, HOX4E, Homeobox protein Hox-5.4, Hox-4.5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Blocking Peptide
LS-E6112 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C159867. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa243-270
Specificity
This HOXD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-270 amino acids from the C-terminal region of human HOXD8.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About HOXD8
HOXD8 belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. P13378 BF030310 CAA33529.1


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Images

Immunofluorescence

Fluorescent image of HUVEC cell stained with HOXD8 Antibody. HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). HOXD8 immunoreactivity is localized to Nucleus significantly.
Fluorescent image of HUVEC cell stained with HOXD8 Antibody. HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). HOXD8 immunoreactivity is localized to Nucleus significantly.

Western blot

HOXD8 Antibody western blot of T47D cell line lysates (35 ug/lane). The HOXD8 antibody detected the HOXD8 protein (arrow).
HOXD8 Antibody western blot of T47D cell line lysates (35 ug/lane). The HOXD8 antibody detected the HOXD8 protein (arrow).

Immunofluorescence

Fluorescent image of HUVEC cell stained with HOXD8 Antibody. HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). HOXD8 immunoreactivity is localized to Nucleus significantly.
Fluorescent image of HUVEC cell stained with HOXD8 Antibody. HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). HOXD8 immunoreactivity is localized to Nucleus significantly.

Western blot

HOXD8 Antibody western blot of T47D cell line lysates (35 ug/lane). The HOXD8 antibody detected the HOXD8 protein (arrow).
HOXD8 Antibody western blot of T47D cell line lysates (35 ug/lane). The HOXD8 antibody detected the HOXD8 protein (arrow).

Immunofluorescence

Fluorescent image of HUVEC cell stained with HOXD8 Antibody. HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). HOXD8 immunoreactivity is localized to Nucleus significantly.
Fluorescent image of HUVEC cell stained with HOXD8 Antibody. HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). HOXD8 immunoreactivity is localized to Nucleus significantly.

Western blot

HOXD8 Antibody western blot of T47D cell line lysates (35 ug/lane). The HOXD8 antibody detected the HOXD8 protein (arrow).
HOXD8 Antibody western blot of T47D cell line lysates (35 ug/lane). The HOXD8 antibody detected the HOXD8 protein (arrow).

Immunofluorescence

Fluorescent image of HUVEC cell stained with HOXD8 Antibody. HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). HOXD8 immunoreactivity is localized to Nucleus significantly.
Fluorescent image of HUVEC cell stained with HOXD8 Antibody. HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). HOXD8 immunoreactivity is localized to Nucleus significantly.

Western blot

HOXD8 Antibody western blot of T47D cell line lysates (35 ug/lane). The HOXD8 antibody detected the HOXD8 protein (arrow).
HOXD8 Antibody western blot of T47D cell line lysates (35 ug/lane). The HOXD8 antibody detected the HOXD8 protein (arrow).

Requested From: United States
Date Requested: 11/13/2018

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