(tested or 100% immunogen sequence identity)
- Western blot (1:1000 - 1:5000)
- ELISA (1:100000)
HMOX1 / HO-1 antibody was raised against a 13 aa peptide sequence within the N-terminus of rat HO-1 (1) was synthesized, coupled to KLH.
The rat sequence is 92% conserved in human and mouse HO-1. Has no significant homology with HO-2. Control peptide, because of the small size (2-3kD), is not recommended for Western. It should be used in ELISA or antibody blocking (use 5-10 ug control peptide per 1 ug of IgG or 1 ul of antiserum) experiments to demonstrate antibody specificity.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for antiserum and 1-10 ug/ml for aff. pure IgG using ECL. HO-1 ~31-33kD. ELISA: ~1:100000 using 50-100 ng control peptide/well.
PBS, 0.05% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.