HO-1 antibody LS-C15674 is an unconjugated rabbit polyclonal antibody to human HO-1 (HMOX1). Validated for IHC and WB.
(tested or 100% immunogen sequence identity)
HMOX1 / HO-1 antibody was raised against a synthetic peptide with the amino acid sequence HDLSEALKEATKEVHC-NH2 corresponding to amino acid residues 12-25 + Cys-NH2 of human HO-1 protein. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Pig, Opossum, Platypus, Xenopus (93%); Turkey, Chicken, Lizard (87%).
Recognizes Heat Shock Protein 32 (Heme Oxygenase-1, HO-1). Species cross-reactivity: Rat spleen, liver and testis, Madin-Darby bovine (MDBK) kidney cells and mouse RAW 264.7 cells following stimulation with gamma interferon and lipopolysaccharide. Does not react with canine (MDCK) kidney cells.
Suitable for use in Western Blot and Immunohistochemistry. Immunohistochemistry: 1:50-1:100 on human airway biopsy, human spleen, human kidney and porcine arteries.
PBS, pH 7.2, 0.01 M sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.