HMGA1 antibody LS-C135054 is an unconjugated rabbit polyclonal antibody to HMGA1 (HMGIY) from human, mouse, rat and other species. Validated for WB.
Human HMGIY / HMGA1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish, Drosophila
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
HMGIY / HMGA1 antibody was raised against synthetic peptide from N-Terminus of human HMGA1 (Q5T6U8, NP_002122). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Drosophila (100%); Opossum (92%); Galago, Chicken (90%); Lizard, Zebrafish (85%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135054. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.