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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HMGIY / HMGA1 Antibody (N‑Terminus) LS‑C135054
HMGA1 antibody LS-C135054 is an unconjugated rabbit polyclonal antibody to HMGA1 (HMGIY) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C135054-50
50 µg
$365
Description
HMGA1 antibody LS-C135054 is an unconjugated rabbit polyclonal antibody to HMGA1 (HMGIY) from human, mouse, rat and other species. Validated for WB.
Target
Human HMGIY / HMGA1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish, Drosophila (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
HMGIY / HMGA1 antibody was raised against synthetic peptide from N-Terminus of human HMGA1 (Q5T6U8, NP_002122). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Drosophila (100%); Opossum (92%); Galago, Chicken (90%); Lizard, Zebrafish (85%).
Blocking Peptide
LS-E13676 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135054. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human HMGA1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About HMGIY / HMGA1

Popular HMGIY / HMGA1 Products

Anti-HMGA1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Dog, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Anti-HMGA1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Dog, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-HMGA1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Dog, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Fluorescent image of A549 cell stained with HMGA1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HMGA1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). HMGA1 immunoreactivity is localized to Nucleus significantly.
Species: Human, Mouse, Rat
Applications: Immunofluorescence, Western blot, Flow Cytometry
Immunohistochemistry of paraffin-embedded human stomach using HMGA1 antibody at dilution of 1:100 (40x lens).
Species: Human
Applications: IHC, Immunofluorescence, Western blot

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Images

Western blot

HMGA1 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGA1 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HMGA1 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGA1 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HMGA1 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGA1 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HMGA1 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGA1 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/20/2018
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