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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HMGIY / HMGA1 Antibody LS-C747780

Immunohistochemistry of paraffin-embedded mouse kidney using HMGA1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HMGA1 antibody at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using HMGA1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Immunohistochemistry of paraffin-embedded mouse kidney using HMGA1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HMGA1 antibody at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using HMGA1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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HMGIY / HMGA1 Antibody LS-C747780

Antibody:
Rabbit Polyclonal to Human HMGIY / HMGA1
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$235 
LS-C747780-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human HMGIY / HMGA1
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HMGA1 antibody LS-C747780 is an unconjugated rabbit polyclonal antibody to HMGA1 (HMGIY) from human, mouse and rat. Validated for IHC and WB.

Target
Human HMGIY / HMGA1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • Western blot (1:1000 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HMGIY / HMGA1 antibody was raised against a synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGA1 (NP_665908.1). MSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRPKKLEKEEEEGISQ
Usage
The predicted MW is 10kDa/11kDa/19kDa, while the observed MW by Western blot was 16kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HMGIY / HMGA1

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Requested From: United States
Date Requested: 7/16/2019
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