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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HLA‑F Antibody LS‑C751644
HLA-F antibody LS-C751644 is an unconjugated rabbit polyclonal antibody to human HLA-F. Validated for ELISA, IHC and WB.
Antibody
Catalog
Size
Price
LS-C751644-100
100 µg
$375

Popular HLA-F Products

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bovine
Applications: Western blot
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Immunofluorescence staining of Hela cells diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Species: Human
Applications: Immunofluorescence, ELISA
Western blot analysis of extracts of A-549 cells, using HLA-F antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
Species: Human, Rat
Applications: Western blot

Product Description

HLA-F antibody LS-C751644 is an unconjugated rabbit polyclonal antibody to human HLA-F. Validated for ELISA, IHC and WB.

Specifications

Target
Human HLA-F
Synonyms
HLA-F, CDA12, HLAF, HLA-CDA12, MHC class I antigen F, HLA F antigen, MHC class Ib antigen, HLA-5.4, Leukocyte antigen F
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified to greater than 95% as determined by SDS-PAGE.
Modifications
Unmodified
Applications
  • IHC (1:20 - 1:200)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C for up to 2 years. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About HLA-F
HLA-F belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. P30511 NM_018950 NP_061823.2


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Requested From: United States
Date Requested: 11/14/2018

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