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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HLA‑F Antibody (aa71‑120, HRP) LS‑C452304
HLA-F antibody LS-C452304 is an HRP-conjugated rabbit polyclonal antibody to HLA-F from human, chimpanzee and monkey. Validated for WB.
Catalog
Size
Price
LS-C452304-100
100 µl (0.65 mg/ml)
$385
Description
HLA-F antibody LS-C452304 is an HRP-conjugated rabbit polyclonal antibody to HLA-F from human, chimpanzee and monkey. Validated for WB.
Target
Human HLA-F
Host
Rabbit
Reactivity
Human, Chimpanzee, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with Biotin, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
HLA-F antibody was raised against synthetic peptide located between aa71-120 of human HLA-F (Q5JQI8, NP_001091949). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey (100%).
Epitope
aa71-120
Specificity
Human HLA-F
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About HLA-F
P30511 NM_018950 NP_061823.2

Popular HLA-F Products

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bovine
Applications: Western blot
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Immunofluorescence staining of Hela cells diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Species: Human
Applications: Immunofluorescence, ELISA
Anti-HLA-F Antibody at 1:2000 dilution + Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Species: Human
Applications: Western blot, ELISA

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Images

Western blot

HLA-F antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HLA-F antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HLA-F antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HLA-F antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HLA-F antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HLA-F antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HLA-F antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HLA-F antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 1/17/2019
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