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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HLA‑DRB4 Antibody LS‑C496790

Catalog Number Size Price
LS-C496790-50 50 µl $235 
LS-C496790-100 100 µl $295 
LS-C496790-200 200 µl $385 
Western blot analysis of extracts of various cell lines, using HLA-DRB4 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.

HLA‑DRB4 Antibody LS‑C496790

Antibody:
Rabbit Polyclonal to Human HLA-DRB4
Application:
WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$235 
LS-C496790-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human HLA-DRB4
Application:
WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
HLA-DRB4 antibody LS-C496790 is an unconjugated rabbit polyclonal antibody to HLA-DRB4 from human and mouse. Validated for WB.
Target
Human HLA-DRB4
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:4000)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-227 of human HLA-DRB4 (NP_068818.4). GDTQPRFLEQAKCECHFLNGTERVWNLIRYIYNQEEYARYNSDLGEYQAVTELGRPDAEYWNSQKDLLERRRAEVDTYCRYNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVNGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSMMSPLTVQWSARSESAQSK
Usage
The predicted MW is 29kDa, while the observed MW by Western blot was 30kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HLA-DRB4
P13762 NM_021983 NP_068818.4

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Requested From: United States
Date Requested: 9/22/2019