Human HLA-A25 + HLA-Aw32
(tested or 100% immunogen sequence identity)
Protein G purified
Also available Unmodified
- IHC - Paraffin (1:1)
- Flow Cytometry
- Applications tested for the base form of this product only
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Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, and DRw10 were used as the immunogen for the HLA-A25 antibody (HLA-Aw32).
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31. B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This mAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32. Optimal dilution of the HLA-A25 antibody (HLA-Aw32) should be determined by the researcher. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
PBS, 0.01% BSA, 0.05% sodium azide
Store at 2°C to 8°C.
For research use only.
This antibody carries the LSBio 100% Guarantee.