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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C390279-7 7 ml $465 
HLA-A25 + HLA-Aw32 Antibody - Formalin-fixed, paraffin-embedded human tonsil stained with HLA-Aw32 / HLA-A25 antibody (CATA-1).  This image was taken for the unmodified form of this product. Other forms have not been tested.

HLA‑A25 + HLA‑Aw32 Antibody (clone CATA‑1, Ready‑to‑Use) LS‑C390279

HLA‑A25 + HLA‑Aw32 Antibody (clone CATA‑1, Ready‑to‑Use) LS‑C390279

Antibody:
Mouse Monoclonal to Human HLA-A25 + HLA-Aw32
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human
Format:
Unconjugated, Ready-to-Use
Other formats:
Price
Catalog Number
$465 
LS-C390279-7
7 ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human HLA-A25 + HLA-Aw32
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human
Format:
Unconjugated, Ready-to-Use
Other formats:

Specifications

Description
HLA-A25 + HLA-Aw32 antibody LS-C390279 is an unconjugated mouse monoclonal antibody to human HLA-A25 + HLA-Aw32. Validated for Flow, IF and IHC.
Target
Human HLA-A25 + HLA-Aw32
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
CATA-1
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Ready-to-Use. Also available Unmodified or Azide-free.
Applications
  • IHC
  • IHC - Paraffin (1:1)
  • Immunofluorescence
  • Flow Cytometry
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, and DRw10 were used as the immunogen for the HLA-A25 antibody (HLA-Aw32).
Specificity
Human HLA-A25+Aw32
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31. B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This mAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32. Optimal dilution of the HLA-A25 antibody (HLA-Aw32) should be determined by the researcher. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Presentation
PBS, 0.01% BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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HLA-A25 + HLA-Aw32 Antibody - Formalin-fixed, paraffin-embedded human tonsil stained with HLA-Aw32 / HLA-A25 antibody (CATA-1).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
HLA-A25 + HLA-Aw32 Antibody - Formalin-fixed, paraffin-embedded human tonsil stained with HLA-Aw32 / HLA-A25 antibody (CATA-1).  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Requested From: United States
Date Requested: 10/17/2019