Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
A synthetic linear peptide corresponding to monomethylated Lys9 at the N-Terminus of Histone H3 (KLH).
Specificity
Recognizes human monomethylated Lys9 at and around the N-terminal of Histone H3, MW ~17kD. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) in Western Blot analysis of HeLa acid extracts and various other modified histone peptides.
Applications
- Western blot (1 µg/ml)
- Functional Assay
Usage
Suitable for use in Western Blot and Peptide Inhibition Assay. Western Blot: 1 ug/ml detects Histone H3 in 500 ng of recombinant Histone H3 and 10 ug of HeLa acid extract.
Presentation
PBS, pH 7.2, 0.05% Sodium Azide
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee