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| Catalog Number | Size | Price |
|---|---|---|
| LS-C745440-50 | 50 µg (0.72 mg/ml) | $582 |
![Histone H3 Antibody - Immunocytochemistry of rabbit Anti-Histone H3 [Monomethyl Lys4] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Monomethyl Lys4] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Monomethyl Lys4] is nuclear and chromosomal. Staining: Histone H3 [Monomethyl Lys4] is expressed in green while the nuclei and aplpha-tubulin were coexpressed with DAPI (blue) and Phalloidin (red).](https://lsbio-7d62.kxcdn.com/image2/histone-h3-antibody-me-lys4-ls-c745440/445914_3396446.jpg)
![Histone H3 Antibody - Western Blot of rabbit Anti-Histone H3 [Monomethyl Lys4] Antibody. Lane 1: HeLa histone prep lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Monomethyl Lys4] at 1:500 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.](https://lsbio-7d62.kxcdn.com/image2/histone-h3-antibody-me-lys4-ls-c745440/445916_3396448.jpg)
![Histone H3 Antibody - Western Blot of rabbit Anti-Histone H3 [Monomethyl Lys4] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Monomethyl Lys4] at 1:500 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.](https://lsbio-7d62.kxcdn.com/image2/histone-h3-antibody-me-lys4-ls-c745440/445915_3551576.jpg)
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Polyclonal Rabbit anti‑Human Histone H3 Antibody (Me‑Lys4, IHC, IF, WB) LS‑C745440
Polyclonal Rabbit anti‑Human Histone H3 Antibody (Me‑Lys4, IHC, IF, WB) LS‑C745440
Antibody:
Histone H3 Rabbit anti-Human Polyclonal (Me-Lys4) Antibody
Application:
IHC, IF, WB, ChrIP
Reactivity:
Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Histone H3 Rabbit anti-Human Polyclonal (Me-Lys4) Antibody
Application:
IHC, IF, WB, ChrIP
Reactivity:
Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
Format:
Unconjugated, Unmodified
Specifications
Description
Histone H3 antibody LS-C745440 is an unconjugated rabbit polyclonal antibody to Histone H3 (Me-Lys4) from human. It is reactive with human, mouse, rat and other species. Validated for ChrIP, IF, IHC and WB.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Histone H3 [Monomethyl Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 4 of human Histone H3.2.
Epitope
Me-Lys4
Specificity
This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Applications
- IHC (1:500)
- Immunofluorescence (1:500)
- Western blot (1:500)
- Chromatin Immunoprecipitation (2 - 5 µg/10E6 cells)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 30% Glycerol
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
ICC, Immunofluorescence, Flow Cytometry, ELISA
Species:
Human, Bovine
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, Western blot, ELISA
Species:
Human
Applications:
IHC, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
