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Catalog Number Size Price
LS-C745012-25 25 µl (1 mg/ml) $275 
Histone H3 Antibody - Western Blot of rabbit anti-Histone H3 K4Me1 antibody. Lane 1: Raji Whole Cell Lysate. Load: 35 µg per lane. Primary antibody: H3K4me1 antibody at 1:2000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15kDa for Histone H3.

Polyclonal Rabbit anti‑Human Histone H3 Antibody (IHC, IF, WB) LS‑C745012

Polyclonal Rabbit anti‑Human Histone H3 Antibody (IHC, IF, WB) LS‑C745012

Antibody:
Histone H3 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, ELISA, ChrIP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745012-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Histone H3 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, ELISA, ChrIP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Histone H3 antibody LS-C745012 is an unconjugated rabbit polyclonal antibody to human Histone H3. Validated for ChrIP, ELISA, IF, IHC and WB.
Target
Human Histone H3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity purified
Modifications
Unmodified
Immunogen
Histone H3 K4me1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 4 of human Histone H3.2.
Specificity
This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Applications
  • IHC (2 - 5 µg/ml)
  • Immunofluorescence (2 - 5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:3000 - 1:10000)
  • Chromatin Immunoprecipitation (2 - 5 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/17/2021