Reactivity
Human, Mouse, Rat, Bovine
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
KLH-conjugated, synthetic peptide (. S[AcK]SAPAPK[AcK]GS[AcK]KAIT[AcK]AQ.) in which AcK corresponds to acetyl-lysine at residues 5, 12, 15 and 20 of human Histone H2B. The immunizing sequence has 24 out of 26 identical amino acids in mouse, rat and bovine.
Epitope
Acetyl-Lys12/Lys15/Lys20
Specificity
Recognizes acetylated Histone H2B, Mr ~15kD. Does not recognize acetylated Histone H2A or unacetylated recombinant Histone H2B protein. Species cross-reactivity: Human. Predicted to cross-react with mouse, rat and bovine based on sequence homology.
Usage
Suitable for use in Western Blot. Western Blot Analysis: 1:40000-1:80000 dilution of this lot detected acetylated Histone H2B in sodium butyrate treated, acid extracted proteins from HeLa cells. Unacetylated recombinant Histone H2B was not detected.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee