Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Hyper-phosphorylated isoform of Tetrahymena Histone H1.
Epitope
Hyperphosphorylated
Specificity
Recognizes phosphorylated histone H1 (32kD). Additional proteins (Mr 66 and 100kD) were detected. Species cross-reactivity: human, bovine.
Applications
- ICC (5 - 10 µg/ml)
- Western blot (1 - 2 µg/ml)
Usage
Suitable for use in Western Blot and Immunocytochemistry. Proteins were visualized using a goat anti-rabbit secondary antibody labeled with HRP. A chemiluminescence detection system was used. Immunocytochemistry: 5-10 ug/ml showed positive immunostaining for Histone H1 in mitotic HeLa cells fixed with 95% ethanol, 5% glacial acetic acid.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee