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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C745499-50 50 µg (0.69 mg/ml) $455 
HIST4H4 Antibody - Immunofluorescence of Histone H4 K12-Ac (RABBIT) Antibody: Histone H4 K12-Ac antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
HIST4H4 Antibody - Western Blot of Histone H4 K12-Ac (RABBIT) Antibody. Western Blot analysis against untreated cell extracts. Lane 1: HeLa cell lysates. Load: 35 µg per lane. Primary antibody: Histone H4 K12-Ac antibody at 1.0 µg/mL for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 11 kDa for Histone H4 K12-Ac (RABBIT) Antibody. Other band(s): none.
HIST4H4 Antibody - Dot Blot of rabbit Histone H4 K12-Ac (RABBIT) Antibody. Lane 1: K12 unmodified. Lane 2: K12-Acetylated. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H4 K12-Ac antibody at 1:1000 for 45 min at 4°C. Secondary antibody: RABBIT IgG (H&L) Secondary Antibody HRP Conjugated at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 minutes at RT.
HIST4H4 Antibody - Immunofluorescence of Histone H4 K12-Ac (RABBIT) Antibody: Histone H4 K12-Ac antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
HIST4H4 Antibody - Western Blot of Histone H4 K12-Ac (RABBIT) Antibody. Western Blot analysis against untreated cell extracts. Lane 1: HeLa cell lysates. Load: 35 µg per lane. Primary antibody: Histone H4 K12-Ac antibody at 1.0 µg/mL for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 11 kDa for Histone H4 K12-Ac (RABBIT) Antibody. Other band(s): none.
HIST4H4 Antibody - Dot Blot of rabbit Histone H4 K12-Ac (RABBIT) Antibody. Lane 1: K12 unmodified. Lane 2: K12-Acetylated. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H4 K12-Ac antibody at 1:1000 for 45 min at 4°C. Secondary antibody: RABBIT IgG (H&L) Secondary Antibody HRP Conjugated at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 minutes at RT.
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Polyclonal Rabbit anti‑Human HIST4H4 Antibody (Ac‑Lys12, IHC, IF, WB) LS‑C745499

Polyclonal Rabbit anti‑Human HIST4H4 Antibody (Ac‑Lys12, IHC, IF, WB) LS‑C745499

Antibody:
HIST4H4 Rabbit anti-Human Polyclonal (Ac-Lys12) Antibody
Application:
IHC, IF, WB, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$455
LS-C745499-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
HIST4H4 Rabbit anti-Human Polyclonal (Ac-Lys12) Antibody
Application:
IHC, IF, WB, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HIST4H4 antibody LS-C745499 is an unconjugated rabbit polyclonal antibody to human HIST4H4 (Ac-Lys12). Validated for DB, IF, IHC and WB.
Target
Human HIST4H4
Synonyms
HIST4H4 | HIST2H4 | Histone 4, H4 | Histone cluster 4, H4 | Histone H4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Histone H4 [ac Lys12] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 12 of human Histone H4.
Epitope
Ac-Lys12
Specificity
A BLAST analysis was used to suggest cross-reactivity with Alfalfa based on 100% sequence homology. Cross-reactivity with Histone H4 [ac Lys12] from other sources has not been determined.
Applications
  • IHC (1:100)
  • Immunofluorescence (1:10)
  • Western blot (1 µg/ml)
  • Dot Blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST4H4

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Requested From: United States
Date Requested: 9/21/2020