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Catalog Number Size Price
LS-C745499-50 50 µg (0.69 mg/ml) $455 
HIST4H4 Antibody - Immunofluorescence of Histone H4 K12-Ac (RABBIT) Antibody: Histone H4 K12-Ac antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
HIST4H4 Antibody - Western Blot of Histone H4 K12-Ac (RABBIT) Antibody. Western Blot analysis against untreated cell extracts. Lane 1: HeLa cell lysates. Load: 35 µg per lane. Primary antibody: Histone H4 K12-Ac antibody at 1.0 µg/mL for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 11 kDa for Histone H4 K12-Ac (RABBIT) Antibody. Other band(s): none.
HIST4H4 Antibody - Dot Blot of rabbit Histone H4 K12-Ac (RABBIT) Antibody. Lane 1: K12 unmodified. Lane 2: K12-Acetylated. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H4 K12-Ac antibody at 1:1000 for 45 min at 4°C. Secondary antibody: RABBIT IgG (H&L) Secondary Antibody HRP Conjugated at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 minutes at RT.
HIST4H4 Antibody - Immunofluorescence of Histone H4 K12-Ac (RABBIT) Antibody: Histone H4 K12-Ac antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
HIST4H4 Antibody - Western Blot of Histone H4 K12-Ac (RABBIT) Antibody. Western Blot analysis against untreated cell extracts. Lane 1: HeLa cell lysates. Load: 35 µg per lane. Primary antibody: Histone H4 K12-Ac antibody at 1.0 µg/mL for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 11 kDa for Histone H4 K12-Ac (RABBIT) Antibody. Other band(s): none.
HIST4H4 Antibody - Dot Blot of rabbit Histone H4 K12-Ac (RABBIT) Antibody. Lane 1: K12 unmodified. Lane 2: K12-Acetylated. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H4 K12-Ac antibody at 1:1000 for 45 min at 4°C. Secondary antibody: RABBIT IgG (H&L) Secondary Antibody HRP Conjugated at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 minutes at RT.
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HIST4H4 Antibody LS‑C745499

HIST4H4 Antibody LS‑C745499

Antibody:
Rabbit Polyclonal to Human HIST4H4
Application:
IHC, IF, WB, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$455 
LS-C745499-50
50 µg
0.69 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human HIST4H4
Application:
IHC, IF, WB, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HIST4H4 antibody LS-C745499 is an unconjugated rabbit polyclonal antibody to human HIST4H4. Validated for DB, IF, IHC and WB.
Target
Human HIST4H4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • IHC (1:100)
  • Immunofluorescence (1:10)
  • Western blot (1 µg/ml)
  • Dot Blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Histone H4 [ac Lys12] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic aceylated peptide surrounding Lysine 12 of human Histone H4.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST4H4

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HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [p Ser1] Antibody. Tissue: Nonmitotic and telophase HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [p Ser1] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [p Ser1] is nuclear. Staining: Histone H4 [p Ser1] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Chromatin Immunoprecipitation, Dot Blot
HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [Monomethyl Lys20] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Monomethyl Lys20] antibody at a 1:500 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [Monomethyl Lys20] is nuclear and chromosomal. Staining: Histone H4 [Monomethyl Lys20] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
Species: Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
Applications: IHC, Immunofluorescence, Western blot, Chromatin Immunoprecipitation, Dot Blot
HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Dimethyl Lys20] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [Dimethyl Lys20] is nuclear and chromosomal. Staining: Histone H4 [Dimethyl Lys20] is expressed in green, nuclei are counterstained with DAPI (blue).
Species: Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
Applications: IHC, Immunofluorescence, Western blot, Chromatin Immunoprecipitation, Dot Blot
HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [ac Lys5] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [ac Lys5] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [ac Lys5] is nuclear and chromosomal. Staining: Histone H4 [ac Lys5] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Chromatin Immunoprecipitation
HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [ac Lys8] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [ac Lys8] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [ac Lys8] is nuclear. Staining: Histone H4 [ac Lys8] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
Species: Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
Applications: IHC, Immunofluorescence, Western blot, Chromatin Immunoprecipitation, Dot Blot

Requested From: United States
Date Requested: 10/22/2019