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Catalog Number Size Price
LS-C745485-50 50 µg (0.53 mg/ml) $455 
HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Dimethyl Lys20] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [Dimethyl Lys20] is nuclear and chromosomal. Staining: Histone H4 [Dimethyl Lys20] is expressed in green, nuclei are counterstained with DAPI (blue).
HIST4H4 Antibody - Western Blot of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Lane 1: HeLa histone preps. Load: 30 µg per lane. Primary antibody: Histone H4 [Dimethyl Lys20] at 1:2000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
HIST4H4 Antibody - Western Blot of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H4 [Dimethyl Lys20] at 1:2000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
HIST4H4 Antibody - Dot Blot of rabbit Histone H4 [Dimethyl Lys20] Antibody. Lane 1: K9-K14. Lane 2: K9-Kac-K14-Kac. Lane 3: K9-K14-Kac. Lane 4: K9-ac-K14. Load: 0.3, 1, 3, 10, and 30 picomoles of peptide. Primary antibody: Histone H4 [Dimethyl Lys20] antibody at 1:2000 for 45 min at 4°C. Secondary antibody: DyLight 488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
HIST4H4 Antibody - Chromatin Immunoprecipitation of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H4 K20me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Dimethyl Lys20] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [Dimethyl Lys20] is nuclear and chromosomal. Staining: Histone H4 [Dimethyl Lys20] is expressed in green, nuclei are counterstained with DAPI (blue).
HIST4H4 Antibody - Western Blot of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Lane 1: HeLa histone preps. Load: 30 µg per lane. Primary antibody: Histone H4 [Dimethyl Lys20] at 1:2000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
HIST4H4 Antibody - Western Blot of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H4 [Dimethyl Lys20] at 1:2000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
HIST4H4 Antibody - Dot Blot of rabbit Histone H4 [Dimethyl Lys20] Antibody. Lane 1: K9-K14. Lane 2: K9-Kac-K14-Kac. Lane 3: K9-K14-Kac. Lane 4: K9-ac-K14. Load: 0.3, 1, 3, 10, and 30 picomoles of peptide. Primary antibody: Histone H4 [Dimethyl Lys20] antibody at 1:2000 for 45 min at 4°C. Secondary antibody: DyLight 488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
HIST4H4 Antibody - Chromatin Immunoprecipitation of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H4 K20me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
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HIST4H4 Antibody LS‑C745485

HIST4H4 Antibody LS‑C745485

Antibody:
Rabbit Polyclonal to Human HIST4H4
Application:
IHC, IF, WB, ChrIP, DB
Reactivity:
Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
Format:
Unconjugated, Unmodified
Price
Catalog Number
$455 
LS-C745485-50
50 µg
0.53 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human HIST4H4
Application:
IHC, IF, WB, ChrIP, DB
Reactivity:
Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
Format:
Unconjugated, Unmodified

Specifications

Description
HIST4H4 antibody LS-C745485 is an unconjugated rabbit polyclonal antibody to HIST4H4 from human, mouse, rat and other species. Validated for ChrIP, DB, IF, IHC and WB.
Target
Human HIST4H4
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • IHC (1:50)
  • Immunofluorescence (1:50)
  • Western blot (1:2000)
  • Chromatin Immunoprecipitation
  • Dot Blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Histone H4 [Dimethyl Lys20] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 20 of human Histone H4.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% Sodium Azide, 30% Glycerol
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST4H4

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Featured Products

HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [p Ser1] Antibody. Tissue: Nonmitotic and telophase HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [p Ser1] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [p Ser1] is nuclear. Staining: Histone H4 [p Ser1] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Chromatin Immunoprecipitation, Dot Blot
HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [Monomethyl Lys20] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Monomethyl Lys20] antibody at a 1:500 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [Monomethyl Lys20] is nuclear and chromosomal. Staining: Histone H4 [Monomethyl Lys20] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
Species: Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
Applications: IHC, Immunofluorescence, Western blot, Chromatin Immunoprecipitation, Dot Blot
HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [ac Lys5] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [ac Lys5] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [ac Lys5] is nuclear and chromosomal. Staining: Histone H4 [ac Lys5] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Chromatin Immunoprecipitation
HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [ac Lys8] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [ac Lys8] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [ac Lys8] is nuclear. Staining: Histone H4 [ac Lys8] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
Species: Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
Applications: IHC, Immunofluorescence, Western blot, Chromatin Immunoprecipitation, Dot Blot

Requested From: United States
Date Requested: 10/14/2019