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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C745485-50 50 µg (0.53 mg/ml) $455 
HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Dimethyl Lys20] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [Dimethyl Lys20] is nuclear and chromosomal. Staining: Histone H4 [Dimethyl Lys20] is expressed in green, nuclei are counterstained with DAPI (blue).
HIST4H4 Antibody - Western Blot of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Lane 1: HeLa histone preps. Load: 30 µg per lane. Primary antibody: Histone H4 [Dimethyl Lys20] at 1:2000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
HIST4H4 Antibody - Western Blot of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H4 [Dimethyl Lys20] at 1:2000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
HIST4H4 Antibody - Dot Blot of rabbit Histone H4 [Dimethyl Lys20] Antibody. Lane 1: K9-K14. Lane 2: K9-Kac-K14-Kac. Lane 3: K9-K14-Kac. Lane 4: K9-ac-K14. Load: 0.3, 1, 3, 10, and 30 picomoles of peptide. Primary antibody: Histone H4 [Dimethyl Lys20] antibody at 1:2000 for 45 min at 4°C. Secondary antibody: DyLight 488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
HIST4H4 Antibody - Chromatin Immunoprecipitation of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H4 K20me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Dimethyl Lys20] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [Dimethyl Lys20] is nuclear and chromosomal. Staining: Histone H4 [Dimethyl Lys20] is expressed in green, nuclei are counterstained with DAPI (blue).
HIST4H4 Antibody - Western Blot of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Lane 1: HeLa histone preps. Load: 30 µg per lane. Primary antibody: Histone H4 [Dimethyl Lys20] at 1:2000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
HIST4H4 Antibody - Western Blot of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H4 [Dimethyl Lys20] at 1:2000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
HIST4H4 Antibody - Dot Blot of rabbit Histone H4 [Dimethyl Lys20] Antibody. Lane 1: K9-K14. Lane 2: K9-Kac-K14-Kac. Lane 3: K9-K14-Kac. Lane 4: K9-ac-K14. Load: 0.3, 1, 3, 10, and 30 picomoles of peptide. Primary antibody: Histone H4 [Dimethyl Lys20] antibody at 1:2000 for 45 min at 4°C. Secondary antibody: DyLight 488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
HIST4H4 Antibody - Chromatin Immunoprecipitation of rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H4 K20me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
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Polyclonal Rabbit anti‑Human HIST4H4 Antibody (Me2‑Lys20, IHC, IF, WB) LS‑C745485

Polyclonal Rabbit anti‑Human HIST4H4 Antibody (Me2‑Lys20, IHC, IF, WB) LS‑C745485

Antibody:
Rabbit Polyclonal to Human HIST4H4
Application:
IHC, IF, WB, ChrIP, DB
Reactivity:
Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
Format:
Unconjugated, Unmodified
Price
Catalog Number
$455 
LS-C745485-50
50 µg
0.53 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human HIST4H4
Application:
IHC, IF, WB, ChrIP, DB
Reactivity:
Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
Format:
Unconjugated, Unmodified

Specifications

Description
HIST4H4 antibody LS-C745485 is an unconjugated rabbit polyclonal antibody to HIST4H4 (Me2-Lys20) from human. It is reactive with human, mouse, rat and other species. Validated for ChrIP, DB, IF, IHC and WB.
Target
Human HIST4H4
Synonyms
HIST4H4 | HIST2H4 | Histone 4, H4 | Histone cluster 4, H4 | Histone H4
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:50)
  • Immunofluorescence (1:50)
  • Western blot (1:2000)
  • Chromatin Immunoprecipitation (2 - 5 µg/10E6 cells)
  • Dot Blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Histone H4 [Dimethyl Lys20] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 20 of human Histone H4.
Epitope
Me2-Lys20
Specificity
This antibody reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 30% Glycerol
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST4H4

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Requested From: United States
Date Requested: 8/8/2020