Products
Services
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C745480-50 50 µg (0.78 mg/ml) $455 
HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [ac Lys5] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [ac Lys5] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [ac Lys5] is nuclear and chromosomal. Staining: Histone H4 [ac Lys5] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
HIST4H4 Antibody - Western Blot of rabbit Anti-Histone H4 [ac Lys5] Antibody. Lane 1: HeLa histone prep. Load: 30 µg per lane. Primary antibody: Histone H4 [ac Lys5] at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
HIST4H4 Antibody - Western Blot of rabbit Anti-Histone H4 [ac Lys5] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 µg per lane. Primary antibody: Histone H4 [ac Lys5] at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
HIST4H4 Antibody - Chromatin Immunoprecipitation of rabbit Anti-Histone H4 [ac Lys5] Antibody. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 ug of Anti-Histone H4 K5ac was used to IP DNA from fixed Hela cells alongside a no antibody control. DNA was measured by qRT-PCR and normalized to total input (input=1).
HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [ac Lys5] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [ac Lys5] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [ac Lys5] is nuclear and chromosomal. Staining: Histone H4 [ac Lys5] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
HIST4H4 Antibody - Western Blot of rabbit Anti-Histone H4 [ac Lys5] Antibody. Lane 1: HeLa histone prep. Load: 30 µg per lane. Primary antibody: Histone H4 [ac Lys5] at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
HIST4H4 Antibody - Western Blot of rabbit Anti-Histone H4 [ac Lys5] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 µg per lane. Primary antibody: Histone H4 [ac Lys5] at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
HIST4H4 Antibody - Chromatin Immunoprecipitation of rabbit Anti-Histone H4 [ac Lys5] Antibody. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 ug of Anti-Histone H4 K5ac was used to IP DNA from fixed Hela cells alongside a no antibody control. DNA was measured by qRT-PCR and normalized to total input (input=1).
1 of 4
2 of 4
3 of 4
4 of 4

Polyclonal Rabbit anti‑Human HIST4H4 Antibody (Ac‑Lys5, IF, WB) LS‑C745480

Polyclonal Rabbit anti‑Human HIST4H4 Antibody (Ac‑Lys5, IF, WB) LS‑C745480

Antibody:
Rabbit Polyclonal to Human HIST4H4
Application:
IF, WB, ChrIP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$455 
LS-C745480-50
50 µg
0.78 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human HIST4H4
Application:
IF, WB, ChrIP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HIST4H4 antibody LS-C745480 is an unconjugated rabbit polyclonal antibody to HIST4H4 (Ac-Lys5) from human. It is reactive with human, mouse and rat. Validated for ChrIP, IF and WB.
Target
Human HIST4H4
Synonyms
HIST4H4 | HIST2H4 | Histone 4, H4 | Histone cluster 4, H4 | Histone H4
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Chromatin Immunoprecipitation (2 - 5 µg/10E6 cells)
Immunogen
Histone H4 [ac Lys5] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 5 of human Histone H4.
Epitope
Ac-Lys5
Specificity
This antibody reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 30% Glycerol
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST4H4

Publications (0)

Customer Reviews (0)


Requested From: United States
Date Requested: 8/11/2020