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Catalog Number Size Price
LS-C745450-50 50 µg (1 mg/ml) $509 
HIST3H3 Antibody - Immunofluorescence of rabbit Anti-Histone H3 [Dimethyl Lys9] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Dimethyl Lys9] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Dimethyl Lys9] is nuclear and chromosomal. Staining: Histone H3 [Dimethyl Lys9] is expressed in green and the nuclei are counterstained with DAPI (blue).
HIST3H3 Antibody - Western Blot of rabbit Anti-Histone H3 [Dimethyl Lys9] Antibody. Lane 1: HeLa histone preps. Load: 30 µg per lane. Primary antibody: Histone H3 [Dimethyl Lys9] at 1:500 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
HIST3H3 Antibody - Western Blot of rabbit Anti-Histone H3 [Dimethyl Lys9] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Dimethyl Lys9] at 1:500 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
HIST3H3 Antibody - Dot Blot of rabbit Histone H3 [Dimethyl Lys9] Antibody. Lane 1: K9 KMe1. Lane 2: K9 KMe2. Lane 3: K9 KMe3. Lane 4: K9. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Dimethyl Lys9] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: DyLight 488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
HIST3H3 Antibody - Chromatin Immunoprecipitation of Histone H3 [Dimethyl Lys9] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H3 K9me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
HIST3H3 Antibody - Immunofluorescence of rabbit Anti-Histone H3 [Dimethyl Lys9] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Dimethyl Lys9] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Dimethyl Lys9] is nuclear and chromosomal. Staining: Histone H3 [Dimethyl Lys9] is expressed in green and the nuclei are counterstained with DAPI (blue).
HIST3H3 Antibody - Western Blot of rabbit Anti-Histone H3 [Dimethyl Lys9] Antibody. Lane 1: HeLa histone preps. Load: 30 µg per lane. Primary antibody: Histone H3 [Dimethyl Lys9] at 1:500 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
HIST3H3 Antibody - Western Blot of rabbit Anti-Histone H3 [Dimethyl Lys9] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Dimethyl Lys9] at 1:500 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
HIST3H3 Antibody - Dot Blot of rabbit Histone H3 [Dimethyl Lys9] Antibody. Lane 1: K9 KMe1. Lane 2: K9 KMe2. Lane 3: K9 KMe3. Lane 4: K9. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Dimethyl Lys9] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: DyLight 488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
HIST3H3 Antibody - Chromatin Immunoprecipitation of Histone H3 [Dimethyl Lys9] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H3 K9me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
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Polyclonal Rabbit anti‑Human HIST3H3 Antibody (Me2‑Lys9, IHC, IF, WB) LS‑C745450

Polyclonal Rabbit anti‑Human HIST3H3 Antibody (Me2‑Lys9, IHC, IF, WB) LS‑C745450

Antibody:
HIST3H3 Rabbit anti-Human Polyclonal (Me2-Lys9) Antibody
Application:
IHC, IF, WB, ChrIP, DB
Reactivity:
Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
Format:
Unconjugated, Unmodified
Price
Catalog Number
$509
LS-C745450-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
HIST3H3 Rabbit anti-Human Polyclonal (Me2-Lys9) Antibody
Application:
IHC, IF, WB, ChrIP, DB
Reactivity:
Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
Format:
Unconjugated, Unmodified

Specifications

Description
HIST3H3 antibody LS-C745450 is an unconjugated rabbit polyclonal antibody to HIST3H3 (Me2-Lys9) from human. It is reactive with human, mouse, rat and other species. Validated for ChrIP, DB, IF, IHC and WB.
Target
Human HIST3H3
Synonyms
HIST3H3 | Histone H3.1t | H3t | Histone 3, H3 | Histone cluster 3, H3
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Histone H3 [Dimethyl Lys9] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 9 of human Histone H3.2.
Epitope
Me2-Lys9
Specificity
This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Applications
  • IHC (1:50)
  • Immunofluorescence (1:50)
  • Western blot (1:500)
  • Chromatin Immunoprecipitation (2 - 5 µg/10E6 cells)
  • Dot Blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 30% Glycerol
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST3H3
Q16695 NM_003493 NP_003484.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/13/2021