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| Catalog Number | Size | Price |
|---|---|---|
| LS-C745450-50 | 50 µg (1 mg/ml) | $582 |
![HIST3H3 Antibody - Immunofluorescence of rabbit Anti-Histone H3 [Dimethyl Lys9] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Dimethyl Lys9] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Dimethyl Lys9] is nuclear and chromosomal. Staining: Histone H3 [Dimethyl Lys9] is expressed in green and the nuclei are counterstained with DAPI (blue).](https://lsbio-7d62.kxcdn.com/image2/hist3h3-antibody-me2-lys9-ls-c745450/445970_3396502.jpg)
![HIST3H3 Antibody - Western Blot of rabbit Anti-Histone H3 [Dimethyl Lys9] Antibody. Lane 1: HeLa histone preps. Load: 30 µg per lane. Primary antibody: Histone H3 [Dimethyl Lys9] at 1:500 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.](https://lsbio-7d62.kxcdn.com/image2/hist3h3-antibody-me2-lys9-ls-c745450/445974_3396506.jpg)
![HIST3H3 Antibody - Western Blot of rabbit Anti-Histone H3 [Dimethyl Lys9] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Dimethyl Lys9] at 1:500 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.](https://lsbio-7d62.kxcdn.com/image2/hist3h3-antibody-me2-lys9-ls-c745450/445971_3551701.jpg)
![HIST3H3 Antibody - Dot Blot of rabbit Histone H3 [Dimethyl Lys9] Antibody. Lane 1: K9 KMe1. Lane 2: K9 KMe2. Lane 3: K9 KMe3. Lane 4: K9. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Dimethyl Lys9] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: DyLight 488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.](https://lsbio-7d62.kxcdn.com/image2/hist3h3-antibody-me2-lys9-ls-c745450/445973_3396505.jpg)
![HIST3H3 Antibody - Chromatin Immunoprecipitation of Histone H3 [Dimethyl Lys9] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H3 K9me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](https://lsbio-7d62.kxcdn.com/image2/hist3h3-antibody-me2-lys9-ls-c745450/445972_3396504.jpg)
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Polyclonal Rabbit anti‑Human HIST3H3 Antibody (Me2‑Lys9, IHC, IF, WB) LS‑C745450
Polyclonal Rabbit anti‑Human HIST3H3 Antibody (Me2‑Lys9, IHC, IF, WB) LS‑C745450
Antibody:
HIST3H3 Rabbit anti-Human Polyclonal (Me2-Lys9) Antibody
Application:
IHC, IF, WB, ChrIP, DB
Reactivity:
Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
HIST3H3 Rabbit anti-Human Polyclonal (Me2-Lys9) Antibody
Application:
IHC, IF, WB, ChrIP, DB
Reactivity:
Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
Format:
Unconjugated, Unmodified
Specifications
Description
HIST3H3 antibody LS-C745450 is an unconjugated rabbit polyclonal antibody to HIST3H3 (Me2-Lys9) from human. It is reactive with human, mouse, rat and other species. Validated for ChrIP, DB, IF, IHC and WB.
Target
Human HIST3H3
Synonyms
HIST3H3 | Histone H3.1t | H3t | Histone 3, H3 | Histone cluster 3, H3
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Xenopus, Drosophila, C. elegans, Plant
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Histone H3 [Dimethyl Lys9] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 9 of human Histone H3.2.
Epitope
Me2-Lys9
Specificity
This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Applications
- IHC (1:50)
- Immunofluorescence (1:50)
- Western blot (1:500)
- Chromatin Immunoprecipitation (2 - 5 µg/10E6 cells)
- Dot Blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 30% Glycerol
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HIST3H3
Publications (0)
Customer Reviews (0)
Featured Products
Reactivity:
Mouse
Range:
0.156-10 ng/ml
Reactivity:
All species
Range:
123.5-10000 pg/ml
Reactivity:
Mouse
Range:
15.6-1000 pg/ml
Reactivity:
All species
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
