Human HIST3H3

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity chromatography

Unmodified

A synthetic methylated peptide corresponding to residues surrounding K4 of human Histone H3 (MonoMethyl K4)

Recognizes endogenous levels of Histone H3 (MonoMethyl K4) protein.

• IHC (1:50 - 1:200)
• IHC - Paraffin
• ICC
• Immunofluorescence (1:50 - 1:200)
• Western blot (1:500 - 1:2000)
• Immunoprecipitation (1:50 - 1:100)
• Chromatin Immunoprecipitation

Western blot analysis of Histone H3 (MonoMethyl K4) expression in Hela (A) whole cell lysates. Immunohistochemical analysis of Histone H3 (MonoMethyl K4) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Immunofluorescent analysis of Histone H3 (MonoMethyl K4) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q16695 NCBI: NM_003493 NP_003484.1