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| Catalog Number | Size | Price |
|---|---|---|
| LS-C745491-50 | 50 µg (0.37 mg/ml) | $567 |
![HIST3H3 Antibody - Western Blot of Histone H3 [ac Lys27] (RABBIT) Antibody. Western Blot analysis against untreated cell extracts. Lane 2: HeLa cell lysates. Lane 3: NIH/3T3 cell lysates. Lane 4: Cos 7 cell lysates. Lane 5: C. Elegans lysates. Load: 35 µg per lane. Primary antibody: Histone H3 [ac Lys27] antibody at 1:400 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 15 kDa for Histone H3 [ac Lys27]. Other band(s): non-specific.](https://lsbio-7d62.kxcdn.com/image2/hist3h3-antibody-ac-lys27-ls-c745491/446108_3396640.jpg)
![HIST3H3 Antibody - Dot Blot of rabbit Histone H3 [ac Lys27] Antibody. Lane 1: K27 unmodified. Lane 2: K27-Ac. Lane 3: K27-Me1. Lane 4: K27-Me2. Lane 5: K27-Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [ac Lys27] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 minutes at RT.](https://lsbio-7d62.kxcdn.com/image2/hist3h3-antibody-ac-lys27-ls-c745491/446107_3396639.jpg)
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Polyclonal Rabbit anti‑Human HIST3H3 Antibody (Ac‑Lys27, WB) LS‑C745491
Polyclonal Rabbit anti‑Human HIST3H3 Antibody (Ac‑Lys27, WB) LS‑C745491
Antibody:
HIST3H3 Rabbit anti-Human Polyclonal (Ac-Lys27) Antibody
Application:
WB, IP, ELISA, DB
Reactivity:
Human, Mouse, Rat, C. elegans
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
HIST3H3 Rabbit anti-Human Polyclonal (Ac-Lys27) Antibody
Application:
WB, IP, ELISA, DB
Reactivity:
Human, Mouse, Rat, C. elegans
Format:
Unconjugated, Unmodified
Specifications
Description
HIST3H3 antibody LS-C745491 is an unconjugated rabbit polyclonal antibody to HIST3H3 (Ac-Lys27) from human. It is reactive with human, mouse, rat and other species. Validated for DB, ELISA, IP and WB.
Target
Human HIST3H3
Synonyms
HIST3H3 | Histone H3.1t | H3t | Histone 3, H3 | Histone cluster 3, H3
Host
Rabbit
Reactivity
Human, Mouse, Rat, C. elegans
(tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, C. elegans (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Histone H3 [ac Lys27] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27ac site of human Histone H3.
Epitope
Ac-Lys27
Specificity
A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [ac Lys27] from other sources has not been determined.
Applications
- Western blot (1:500 - 1:1000)
- Immunoprecipitation (1:100)
- ELISA (1:10000)
- Dot Blot
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 30% Glycerol
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HIST3H3
Publications (0)
Customer Reviews (0)
Featured Products
Reactivity:
Human
Range:
0.78-50 ng/ml
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Reactivity:
Mouse
Range:
2.47-200 ng/ml
Reactivity:
Mouse
Range:
78-5000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
