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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C750204-50 50 µl $265 
LS-C750204-100 100 µl $345 
LS-C750204-200 200 µl $445 
HIST2H4A Antibody - Immunohistochemistry of paraffin-embedded human stomach using H4K5AC antibody at dilution of 1:100 (40x lens).
HIST2H4A Antibody - Immunohistochemistry of paraffin-embedded mouse brain using H4K5AC antibody at dilution of 1:100 (40x lens).
HIST2H4A Antibody - Immunohistochemistry of paraffin-embedded rat brain using H4K5AC antibody at dilution of 1:100 (40x lens).
HIST2H4A Antibody - Immunohistochemistry of paraffin-embedded human tonsil using H4K5AC antibody at dilution of 1:100 (40x lens).
HIST2H4A Antibody - Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody at 1:1000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
HIST2H4A Antibody - Immunohistochemistry of paraffin-embedded human stomach using H4K5AC antibody at dilution of 1:100 (40x lens).
HIST2H4A Antibody - Immunohistochemistry of paraffin-embedded mouse brain using H4K5AC antibody at dilution of 1:100 (40x lens).
HIST2H4A Antibody - Immunohistochemistry of paraffin-embedded rat brain using H4K5AC antibody at dilution of 1:100 (40x lens).
HIST2H4A Antibody - Immunohistochemistry of paraffin-embedded human tonsil using H4K5AC antibody at dilution of 1:100 (40x lens).
HIST2H4A Antibody - Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody at 1:1000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
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HIST2H4A Antibody LS‑C750204

HIST2H4A Antibody LS‑C750204

Antibody:
Rabbit Polyclonal to Human HIST2H4A
Application:
IHC, WB, ChrIP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$265 
LS-C750204-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human HIST2H4A
Application:
IHC, WB, ChrIP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HIST2H4A antibody LS-C750204 is an unconjugated rabbit polyclonal antibody to HIST2H4A from human, mouse and rat. Validated for ChrIP, IHC and WB.
Target
Human HIST2H4A
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
  • Chromatin Immunoprecipitation (1:20 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthetic acetylated peptide around K5 of human Histone H4 (NP_001029249.1). GRGKG
Usage
The predicted MW is 11kDa, while the observed MW by Western blot was 11kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST2H4A

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Requested From: United States
Date Requested: 10/13/2019