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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C676892-50 50 µl $295 
LS-C676892-100 100 µl $395 
HIST1H2BN Antibody - Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Mouse liver tissue All Lanes: HIST1H2BC antibody at 0.92µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 KDa Observed band size: 14 KDa
HIST1H2BN Antibody - Immunoprecipitating HIST1H2BC in MCF-7 whole cell lysate Lane 1: Rabbit control IgG instead of HIST1H2BC (Ab-116) Antibody in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: HIST1H2BC (Ab-116) Antibody (5µg) + MCF-7 whole cell lysate (500µg) Lane 3: MCF-7 whole cell lysate (20µg)
HIST1H2BN Antibody - Chromatin Immunoprecipitation Hela (4*10E6) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (HIST1H2BC (Ab-116) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
HIST1H2BN Antibody - Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Mouse liver tissue All Lanes: HIST1H2BC antibody at 0.92µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 KDa Observed band size: 14 KDa
HIST1H2BN Antibody - Immunoprecipitating HIST1H2BC in MCF-7 whole cell lysate Lane 1: Rabbit control IgG instead of HIST1H2BC (Ab-116) Antibody in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: HIST1H2BC (Ab-116) Antibody (5µg) + MCF-7 whole cell lysate (500µg) Lane 3: MCF-7 whole cell lysate (20µg)
HIST1H2BN Antibody - Chromatin Immunoprecipitation Hela (4*10E6) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (HIST1H2BC (Ab-116) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
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HIST1H2BN Antibody LS‑C676892

HIST1H2BN Antibody LS‑C676892

Antibody:
Rabbit Polyclonal to Human HIST1H2BN
Application:
WB, ELISA, ChrIP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295 
LS-C676892-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human HIST1H2BN
Application:
WB, ELISA, ChrIP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HIST1H2BN antibody LS-C676892 is an unconjugated rabbit polyclonal antibody to human HIST1H2BN. Validated for ChrIP, ELISA and WB.
Target
Human HIST1H2BN
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
  • Chromatin Immunoprecipitation
Immunogen
Peptide sequence around site of Mono-methyl-Lys (116) derived from human Histone H2B
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST1H2BN
Q99877 Q99877

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Requested From: United States
Date Requested: 12/14/2019