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Antibodies > HIST1H2BB > LS-C675313
 
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Catalog Number Size Price
LS-C675313-50 50 µl $316 
LS-C675313-100 100 µl $386 
HIST1H2BB Antibody - Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
HIST1H2BB Antibody - Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) diluted at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
HIST1H2BB Antibody - Western Blot Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min All Lanes:Acetyl-Histone H2B type 1-B (K20) antibody at 0.135µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa
HIST1H2BB Antibody - Overlay histogram showing Hela cells stained with Acetyl-Histone H2B type 1-B Antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4?.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4?. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
HIST1H2BB Antibody - Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
HIST1H2BB Antibody - Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) diluted at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
HIST1H2BB Antibody - Western Blot Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min All Lanes:Acetyl-Histone H2B type 1-B (K20) antibody at 0.135µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa
HIST1H2BB Antibody - Overlay histogram showing Hela cells stained with Acetyl-Histone H2B type 1-B Antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4?.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4?. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Monoclonal Rabbit anti‑Human HIST1H2BB Antibody (IF, WB) LS‑C675313

Monoclonal Rabbit anti‑Human HIST1H2BB Antibody (IF, WB) LS‑C675313

Submit Review LSBio Guarantee
Antibody:
HIST1H2BB Rabbit anti-Human Monoclonal Antibody
Application:
ICC, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$316
LS-C675313-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HIST1H2BB Rabbit anti-Human Monoclonal Antibody
Application:
ICC, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HIST1H2BB antibody LS-C675313 is an unconjugated rabbit monoclonal antibody to human HIST1H2BB. Validated for ELISA, Flow, ICC, IF and WB.
Target
Human HIST1H2BB
Synonyms
HIST1H2BB | H2B.1 | Histone H2B type 1-B | Histone H2B.f | Histone H2b | Histone H2B.1 | Histone 1, H2bb | Histone cluster 1, H2bb
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
A synthesized peptide
Applications
  • ICC
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • ELISA
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST1H2BB
P33778 NM_021062 NP_066406.1

Publications (0)

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HIST1H2BB ELISA Kit
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Reactivity: Human
Range: 0.156-10 ng/ml
HIST1H2BB Protein
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Source: E. coli
Tag: His-SUMO
See all HIST1H2BB Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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