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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HIST1H1E Antibody LS‑C676867
HIST1H1E antibody LS-C676867 is an unconjugated rabbit polyclonal antibody to human HIST1H1E. Validated for ChrIP, ELISA and IHC.
Catalog
Size
Price
LS-C676867-50
50 µl
$295
LS-C676867-100
100 µl
$395

Popular HIST1H1E Products

HIST1H1E antibody Western blot of ACHN lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Dog, Rabbit
Applications: Western blot
Immunohistochemistry of paraffin-embedded mouse colon tissue.
Species: Human
Applications: IHC, ICC, Immunofluorescence, Western blot
Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, ELISA
Immunohistochemistry image of paraffin-embedded human melanoma cancer at a dilution of 1:100
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunohistochemistry image of paraffin-embedded human lung cancer at a dilution of 1:100
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA

Product Description

HIST1H1E antibody LS-C676867 is an unconjugated rabbit polyclonal antibody to human HIST1H1E. Validated for ChrIP, ELISA and IHC.

Specifications

Target
Human HIST1H1E
Synonyms
HIST1H1E, H1.4, Histone cluster 1, H1e, Histone H1b, H1 histone family, member 4, H1s-4, Histone 1 family 4, Histone 1, H1e, Histone H1.4, H1E
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • ELISA
  • Chromatin Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HIST1H1E antibody was raised against peptide sequence around site of B-hydroxybutyryl-Lys (85) derived from human Histone H1.3
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only.
About HIST1H1E
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. P10412 NM_005321 NP_005312.1


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Images

Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:20 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:20 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation Hela(4*106)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1D or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the Beta-Globin promoter.
Chromatin Immunoprecipitation Hela(4*106)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1D or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the Beta-Globin promoter.

Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:20 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:20 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation Hela(4*106)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1D or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the Beta-Globin promoter.
Chromatin Immunoprecipitation Hela(4*106)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1D or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the Beta-Globin promoter.

Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:20 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:20 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation Hela(4*106)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1D or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the Beta-Globin promoter.
Chromatin Immunoprecipitation Hela(4*106)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1D or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the Beta-Globin promoter.

Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:20 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:20 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation Hela(4*106)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1D or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the Beta-Globin promoter.
Chromatin Immunoprecipitation Hela(4*106)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1D or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the Beta-Globin promoter.

Requested From: United States
Date Requested: 11/13/2018

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