ELISA: Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:8,000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction. Appropriate specificity controls were run. Western Blots: 293T cells were transfected, three different constructs of differing sizes. Whole cell extracts were analyzed and the results compared, lysates from non- transfected cells. The anti-HHHHHH was an efffective antibody at a 1:1000 (1 ug/ml) dilution. The Westerns probed, this antibody are very sharp and clear, extremely low background and no non-specific binding, untransfected extracts. Also effective, Invitrogen Positope. Other applications should be user optimized.
PBS, pH 7.2, 0.1 % sodium azide.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only. Intended for use by laboratory professionals.
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