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His Tag Antibody (clone 33D10.D2.G8) LS‑C153999

Catalog Number Size Price
LS-C153999-100 100 µg (1 mg/ml) $345 
Anti-6x-His Epitope Tag Monoclonal Antibody - Western Blot. Western blot of Immunochemicals Mouse Anti-6x-His Epitope Tag Monoclonal Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). In lane 1 are molecular weight markers.

His Tag Antibody (clone 33D10.D2.G8) LS‑C153999

Antibody:
Mouse Monoclonal to His Tag
Application:
WB, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$345 
LS-C153999-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to His Tag
Application:
WB, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
His Tag antibody LS-C153999 is an unconjugated mouse monoclonal antibody to His Tag. Validated for ELISA and WB.
Host
Mouse
Clonality
IgG1 Monoclonal
Clone
33D10.D2.G8
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • ELISA
Immunogen
HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Specificity
6X HIS Epitope Tag antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Usage
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualized with FITC conjugated Mouse anti-HIS tag antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Synthetic
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Applications: Western blot, ELISA
Anti-6X His Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal rabbit.
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Western blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: IHC, Western blot, ELISA
Product
Species:
Applications: IHC, Western blot, ELISA

Requested From: United States
Date Requested: 8/19/2019
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