LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
HIRA Antibody (aa601‑650) LS‑C116044
HIRA antibody LS-C116044 is an unconjugated rabbit polyclonal antibody to HIRA from human, mouse, dog and other species. Validated for WB.
Catalog
Size
Price
LS-C116044-50
50 µg
$365

Popular HIRA Products

Western blot Image
Species: Human
Applications: Western blot
Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using HIRA Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TUPLE1 mAb.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Dot Blot
Antibody
Species: Human, Mouse
Applications: IHC, Immunofluorescence, ELISA
Immunohistochemistry of paraffin-embedded human lung cancer tissue.
Species: Human, Mouse
Applications: IHC, Western blot

Product Description

HIRA antibody LS-C116044 is an unconjugated rabbit polyclonal antibody to HIRA from human, mouse, dog and other species. Validated for WB.

Specifications

Target
Mouse HIRA
Synonyms
HIRA, DGCR1, HIR, TUPLE1, Protein HIRA
Host
Rabbit
Reactivity
Mouse, Human, Dog, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
HIRA antibody was raised against synthetic peptide located between aa601-650 of mouse Hira (Q61666, NP_034565). Percent identity by BLAST analysis: Mouse (100%); Gorilla, Monkey, Rabbit, Guinea pig (92%); Human, Gibbon, Galago, Marmoset, Pig (85%).
Blocking Peptide
LS-E13293 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116044. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa601-650
Specificity
Mouse HIRA
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About HIRA
HIRA is a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. P54198 NM_003325 NP_003316.3


Publications (0)


Customer Reviews (0)


Images

Western blot

HIRA antibody Western blot of Mouse Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HIRA antibody Western blot of Mouse Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HIRA antibody Western blot of Mouse Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HIRA antibody Western blot of Mouse Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HIRA antibody Western blot of Mouse Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HIRA antibody Western blot of Mouse Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HIRA antibody Western blot of Mouse Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HIRA antibody Western blot of Mouse Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/13/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy