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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HINT / HINT1 Antibody (N‑Terminus, HRP) LS‑C459083
HINT1 antibody LS-C459083 is an HRP-conjugated rabbit polyclonal antibody to HINT1 (HINT) from human, mouse, rat and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C459083-100
100 µl (0.65 mg/ml)
$385
Description
HINT1 antibody LS-C459083 is an HRP-conjugated rabbit polyclonal antibody to HINT1 (HINT) from human, mouse, rat and other species. Validated for IHC and WB.
Target
Human HINT / HINT1
Host
Rabbit
Reactivity
Human, Chimpanzee, Orangutan, Monkey, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Gibbon, Drosophila (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with Biotin, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HINT / HINT1 antibody was raised against synthetic peptide from N-Terminus of human HINT1 (P49773, NP_005331). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Pig, Zebra finch, Chicken, Lizard, Xenopus, Catfish, Salmon, Sablefish, Stickleback, Zebrafish (100%); Gibbon, Opossum (92%); Drosophila, Nematode (90%); Beetle (85%).
Epitope
N-Terminus
Specificity
Human HINT1
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
100 mM sodium phosphate, pH 7.6, 150 mM NaCl
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
About HINT / HINT1
P49773 NM_005340 NP_005331.1

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Images

Immunohistochemistry

Anti-HINT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HINT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 10-20%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 10-20%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HINT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HINT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 10-20%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 10-20%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HINT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HINT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 10-20%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 10-20%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HINT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HINT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 10-20%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 10-20%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/18/2018
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