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| Catalog Number | Size | Price |
|---|---|---|
| LS-B501-0.1 | 0.1 ml (1 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human HIF2A / EPAS1 Antibody (clone ep190b, aa535‑631, IHC, WB) LS‑B501
IHC‑plus™ Monoclonal Mouse anti‑Human HIF2A / EPAS1 Antibody (clone ep190b, aa535‑631, IHC, WB) LS‑B501
Note: This antibody replaces LS-C2564, LS-C7475
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
EPAS1 antibody LS-B501 is an unconjugated mouse monoclonal antibody to EPAS1 (HIF2A) (aa535-631) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human HIF2A / EPAS1
Synonyms
EPAS1 | BHLHe73 | ECYT4 | HIF-1-alpha-like factor | HIF2-alpha | HIF2A | EPAS-1 | HIF-2-alpha | HIF-1alpha-like factor | MOP2 | Member of PAS protein 2 | PASD2
Host
Mouse
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
ep190b
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Human HIF-2 alpha amino acids 535-631
Epitope
aa535-631
Specificity
HIF-2 alpha and does not cross-react with HIF-1 alpha.
Applications
- IHC
- IHC - Paraffin (10 - 20 µg/ml)
- Western blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B501 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B501 was determined to be 10-20 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 1% BSA
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HIF2A / EPAS1
Validation
Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ HIF2A / EPAS1 Antibody (aa535-631, clone ep190b) for IHC, WB/Western LS-B501 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Silencing of int6 gene restores function of the ischaemic hindlimb in a rat model of peripheral arterial disease. Okamoto N, Tanaka A, Jung K, Karasawa K, Orito K, Matsuda A, Amagai Y, Oida K, Ohmori K, Matsuda H. Cardiovascular research. 2011 Nov;92:209-17.
Mitochondrial respiration and redox coupling in articular chondrocytes. Lane RS, Fu Y, Matsuzaki S, Kinter M, Humphries KM, Griffin TM. Arthritis research & therapy. 2015 17:54. (WB; Human)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat, Hamster, Sheep, Fish, Primate
Applications:
IHC, IHC - Paraffin
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
