Human HGF / Hepatocyte Growth Factor

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

mh010

Unconjugated

Protein A purified

Preservative Free

Recombinant Human HGF / Hepatocyte Growth Factor Protein

Human HGF. No cross-reactivity with Mouse HGF or Rat HGF in ELISA assay

• ELISA (2 µg/ml)
• Blocking (4 µg/ml)
• Neutralization

Block: In a functional ELISA which immobilized Human HGF at 2ug/ml (100 µL/well) in the plate, the Mouse and Human Chimeric anti-Human Monoclonal Antibody can block the binding of 4 ug/ml of recombinant Human HGFR to human HGF, the EC50 is 0.75 ug/ml. Neutralization: The neutralization activity of HGF Neutralizing Antibody is Measured by its ability to neutralize autocrine HGF-induced proliferation in the U87 MG cell line. Proliferation of U87 MG cells elicited by autocrine HGF was inhibited by increasing concentrations of Human HGF Monoclonal Antibody. The IC50 is typically 1-4 ug/ml. Please Note: Optimal concentrations/dilutions should be determined by the end user.

0.2 µm filtered solution in PBS

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P14210 NCBI: X16323 CAA34387.1