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Catalog Number Size Price
LS-C36653-100 100 µg $793 
Antibody

Polyclonal Sheep anti‑Human HES4 Antibody (aa94‑221, WB) LS‑C36653

Polyclonal Sheep anti‑Human HES4 Antibody (aa94‑221, WB) LS‑C36653

Antibody:
HES4 Sheep anti-Human Polyclonal (aa94-221) Antibody
Application:
ICC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$793
LS-C36653-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
HES4 Sheep anti-Human Polyclonal (aa94-221) Antibody
Application:
ICC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HES4 antibody LS-C36653 is an unconjugated sheep polyclonal antibody to human HES4 (aa94-221). Validated for ELISA, ICC and WB.
Target
Human HES4
Synonyms
HES4 | BHLHb42 | Transcription factor HES-4 | BHLH factor Hes4 | Hairy and enhancer of split 4 | HHES4
Host
Sheep
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived rhHES-4 (aa 94-221).
Epitope
aa94-221
Specificity
Selected for its ability to recognize human HES-4 in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 1% cross-reactivity with rhHES-1, rhHES-2, rhHES-5, and rhHES-7.
Applications
  • ICC
  • Western blot
  • ELISA
Usage
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 ug/ml to detect HES-4 in HEK293 cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 0.2 ng/well.
Presentation
Lyophilized
Reconstitution
Reconstitute in deionized water
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HES4
Q9HCC6 NM_021170 NP_066993.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/27/2021