Virus Hepatitis B x Antigen (HBxAg)

Mouse

Virus (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

3F6-G10

Unconjugated

Affinity purified

Unmodified

HB-Xag-Protein A Fusion protein.

recognizes the HB-X antigen of hepatitis virus. The Hepatitis B X antigen is a 154 amino acid ~17 kD multifunctional protein involved in the development of liver cirrhosis and hepatocellular carcinoma. Clone 3F6-G10 was produced by immunization of mice with a "HB-X- Protein A" fusion construct and subsequent screening of hybridoma products against a "HB-X-GST" fusion construct. Has been used successfully for the detection of the hepatitis B X antigen by immunohistochemistry in formalin fixed, paraffin embedded material, also by western blotting against the immunizing and screening fusion proteins. Subsequently clone 3F6-G10 has been used as a capture reagent in a sensitive sandwich ELISA and bead based flow assay for the quantitative assessment of HbX antigen in human sera. Fine epitope mapping by phage library screening indicates that the epitope recognized bylies between amino acids 88 and 93 of the X antigen, a result subsequently confirmed by peptide ELISA.

• IHC - Paraffin (1:100)
• IHC - Frozen (1:100)
• ELISA

Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

0.09% Sodium Azide, 0.1% BSA

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

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