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Catalog Number Size Price
LS-C744869-25 25 µl (1 mg/ml) $235 
Hemoglobin C Antibody - Western Blot of Mouse Anti-Hemoglobin beta C Antibody. Lane 1: Molecular Weight Ladder. Lane 2: HbA peptide conjugated to BSA. Lane 3: HbA-2 peptide conjugated to BSA. Lane 4: HbC peptide conjugated to BSA. Lane 5: HbF peptide conjugated to BSA. Lane 6: HbS peptide conjugated to BSA. Lane 7: BSA alone. Load: 50ng per lane. Primary antibody: Anti-HbC antibody at 1µg/mL overnight at 4°C. Secondary antibody: Rabbit Anti-Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-073 for 30 min RT. Predicted/Observed: Reactivity seen in Lane 4 specific to HbC only.
Hemoglobin C Antibody - ELISA results of purified Monoclonal anti-HbC antibody tested against BSA-conjugated peptide of HbC peptide. (Purple line). Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer
Hemoglobin C Antibody - Western Blot of Mouse Anti-Hemoglobin beta C Antibody. Lane 1: Molecular Weight Ladder. Lane 2: HbA peptide conjugated to BSA. Lane 3: HbA-2 peptide conjugated to BSA. Lane 4: HbC peptide conjugated to BSA. Lane 5: HbF peptide conjugated to BSA. Lane 6: HbS peptide conjugated to BSA. Lane 7: BSA alone. Load: 50ng per lane. Primary antibody: Anti-HbC antibody at 1µg/mL overnight at 4°C. Secondary antibody: Rabbit Anti-Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-073 for 30 min RT. Predicted/Observed: Reactivity seen in Lane 4 specific to HbC only.
Hemoglobin C Antibody - ELISA results of purified Monoclonal anti-HbC antibody tested against BSA-conjugated peptide of HbC peptide. (Purple line). Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer
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Hemoglobin C Antibody LS‑C744869

Hemoglobin C Antibody LS‑C744869

Antibody:
Mouse Monoclonal to Human Hemoglobin C
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$235 
LS-C744869-25
25 µl
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human Hemoglobin C
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Hemoglobin C antibody LS-C744869 is an unconjugated mouse monoclonal antibody to human Hemoglobin C. Validated for ELISA and WB.
Target
Human Hemoglobin C
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • ELISA (1:20000)
Immunogen
Anti-Hemoglobin beta C Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb ß-subunit conjugated to KLH.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

Hemoglobin C Antibody - Western Blot of Mouse Anti-Hemoglobin beta C Antibody. Lane 1: Molecular Weight Ladder. Lane 2: HbA peptide conjugated to BSA. Lane 3: HbA-2 peptide conjugated to BSA. Lane 4: HbC peptide conjugated to BSA. Lane 5: HbF peptide conjugated to BSA. Lane 6: HbS peptide conjugated to BSA. Lane 7: BSA alone. Load: 50ng per lane. Primary antibody: Anti-HbC antibody at 1µg/mL overnight at 4°C. Secondary antibody: Rabbit Anti-Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-073 for 30 min RT. Predicted/Observed: Reactivity seen in Lane 4 specific to HbC only.
Species: Human
Applications: Western blot, ELISA
Hemoglobin C ELISA Kit
Reactivity: Human
Range: 0.313-20 ng/ml
Hemoglobin C ELISA Kit
Reactivity: Human
Range: 1.563-100 ng/ml
Hemoglobin C ELISA Kit
Reactivity: Monkey
Range: 1.563-100 ng/ml
Hemoglobin C ELISA Kit
Reactivity: Chicken
Range: 1.563-100 ng/ml
Hemoglobin C ELISA Kit
Reactivity: Porcine
Range: 1.563-100 ng/ml

Requested From: United States
Date Requested: 10/18/2019