Target
Hemagglutinin / HA Tag
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic peptide containing the sequence YPYDVPDYAASL derived from the influenza virus hemaglutinin (HA) protein (KLH coupled). Cellular Localization: Depends upon the localization of the parent protein tagged with the HA tag.
Specificity
Recognizes recombinant proteins containing the HA epitope tag: YPYDVPDYA. Recognizes the HA-tag fused to either the N- or C-termini of targeted proteins. Species cross-reactivity: A broad species cross-reactivity is expected based on conservation of sequence homology.
Applications
- Western blot (5 - 10 µg/ml)
- Immunoprecipitation
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 5-10 ug/ml for 2hrs at RT. Immunoprecipitation: 10 ug/mg protein lysate. Use Protein A. Positive control: Proteins displaying the YPYDVPDYA HA-tag.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 0.2% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee