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Catalog Number	Size	Price
LS-C742395-100	100 µg (0.5 mg/ml)	$503

HDAC2 Antibody - Goat anti-Hdac2 (mouse) Antibody Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

HDAC2 Antibody - Hdac2 antibody immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

HDAC2 Antibody - Goat anti-Hdac2 (mouse) Antibody (1µg/ml) staining of HEK293 nuclear cell lysate. (35µg protein in RIPA buffer). Detected by chemiluminescencence.

HDAC2 Antibody - Hdac2 antibody (1µg/ml) staining of HEK293 nuclear cell lysate. (35µg protein in RIPA buffer). Detected by chemiluminescence.

HDAC2 Antibody - Goat anti-Hdac2 (mouse) Antibody Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

HDAC2 Antibody - Hdac2 antibody flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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Polyclonal Goat anti‑Human HDAC2 Antibody (Internal Region, IF, WB) LS‑C742395

Name: Anti-HDAC2 Antibody | Goat anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C742395-100
Price: 503.0000 USD
Availability: InStock

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Antibody:

HDAC2 Goat anti-Human Polyclonal (Internal Region) Antibody

Application:

IF, WB, Peptide-ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$503

LS-C742395-100

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

HDAC2 Goat anti-Human Polyclonal (Internal Region) Antibody

Application:

IF, WB, Peptide-ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

HDAC2 antibody LS-C742395 is an unconjugated goat polyclonal antibody to human HDAC2 (Internal Region). Validated for IF, Peptide-ELISA and WB.

Target

Human HDAC2

Synonyms

HDAC2 | HD2 | Histone deacetylase 2 | RPD3 | YAF1 | YY1-associated factor 1

See All HDAC2 Antibodies

Host

Goat

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence C-PEDAVHEDSGDE, from the internal region of the protein sequence according to NP_032255.2.

Epitope

Internal Region

Specificity

This antibody imay cross-react with HDAC1

Applications

Immunofluorescence (10 µg/ml)
Western blot (1 - 2 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:2000)

Usage

Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Approx 55-60kDa band observed in nuclear lysates of cell line HEK293 (calculated MW of 55.3kDa according to NP_032255.2). Recommended concentration: 1-2 ug/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the nucleus of U251 cells. Recommended concentration: 10 ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10 ug/ml.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About HDAC2

NCBI: NM_001527 NP_001518.3