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Catalog Number Size Price
LS-C407842-10 10 µg $318 
LS-C407842-100 100 µg $470 
HDAC11 Antibody - HDAC11 antibody IHC-paraffin. IHC(P): Rat Brain Tissue.
HDAC11 Antibody - HDAC11 antibody IHC-paraffin. IHC(P): Mouse Brain Tissue.
HDAC11 Antibody - Western blot analysis of HDAC11 using anti-HDAC11 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC11 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HDAC11 at approximately 40KD. The expected band size for HDAC11 is at 40KD.
HDAC11 Antibody - HDAC11 antibody Western blot. All lanes: Anti HDAC11 at 0.5 ug/ml. Lane 1: Rat Skeletal Muscle Tissue Lysate at 50 ug. Lane 2: Rat Kidney Tissue Lysate at 50 ug. Lane 3: Rat Cardiac Muscle Tissue Lysate at 50 ug. Lane 4: NRK Whole Cell Lysate at 40 ug. Lane 5: 293T Whole Cell Lysate at 40 ug. Lane 6: HELA Whole Cell Lysate at 40 ug. Lane 7: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 39 kD. Observed band size: 39 kD.
HDAC11 Antibody - HDAC11 antibody IHC-paraffin. IHC(P): Rat Brain Tissue.
HDAC11 Antibody - HDAC11 antibody IHC-paraffin. IHC(P): Mouse Brain Tissue.
HDAC11 Antibody - Western blot analysis of HDAC11 using anti-HDAC11 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC11 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HDAC11 at approximately 40KD. The expected band size for HDAC11 is at 40KD.
HDAC11 Antibody - HDAC11 antibody Western blot. All lanes: Anti HDAC11 at 0.5 ug/ml. Lane 1: Rat Skeletal Muscle Tissue Lysate at 50 ug. Lane 2: Rat Kidney Tissue Lysate at 50 ug. Lane 3: Rat Cardiac Muscle Tissue Lysate at 50 ug. Lane 4: NRK Whole Cell Lysate at 40 ug. Lane 5: 293T Whole Cell Lysate at 40 ug. Lane 6: HELA Whole Cell Lysate at 40 ug. Lane 7: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 39 kD. Observed band size: 39 kD.
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Polyclonal Rabbit anti‑Human HDAC11 Antibody (aa47‑81, IHC, WB) LS‑C407842

Polyclonal Rabbit anti‑Human HDAC11 Antibody (aa47‑81, IHC, WB) LS‑C407842

Antibody:
HDAC11 Rabbit anti-Human Polyclonal (aa47-81) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407842-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HDAC11 Rabbit anti-Human Polyclonal (aa47-81) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HDAC11 antibody LS-C407842 is an unconjugated rabbit polyclonal antibody to HDAC11 (aa47-81) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human HDAC11
Synonyms
HDAC11 | HD11 | Histone deacetylase 11
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human HDAC11 (47-81 aa NFLKEEKLLSDSMLVEAREASEEDLLVVHTRRYLN), different from the related mouse sequence by one amino acid.
Epitope
aa47-81
Specificity
Weakly expressed in most tissues. Strongly expressed in brain, heart, skeletal muscle, kidney and testis.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HDAC11
Q96DB2 NM_024827 NP_079103.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/23/2024