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Antibodies > hCG / Chorionic Gonadotropin > LS-C355424
 
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Catalog Number Size Price
LS-C355424-1 1 mg $857 
hCG / Chorionic Gonadotropin Antibody - Immunohistochemistry analysis of hCG was performed on human placenta tissue and is showing positive staining in extra cellular spaces. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with hCG monoclonal antibody at a dilution of 1:20, overnight at 4C in a humidified chamber without (left panel) or with hCG antibody control (right panel). Tissues were washed, and incubated with secondary antibody (conjugated with HRP) for 30 min at room temperature in a humidified chamber. Detection was performed using DAB substrate kit. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting. Images were taken at 20x magnification.
hCG / Chorionic Gonadotropin Antibody - Immunoprecipitation of hCG proteins was performed by spiking 5 ugs of purified hCG protein extracted from human urine into 1ml of 1:2 diluted human urine sample. Antigen-antibody complexes were formed by incubating with 5ug of hCG antibody overnight on end over end tube rotator at 4C. The immune complexes were captured by using 50ul Protein A/G Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% milk for at least 1 hour at room temperature. hCG was detected at ~32 and 22 kD using hCG monoclonal antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
hCG / Chorionic Gonadotropin Antibody - Direct ELISA analysis of hCG proteins was performed by coating wells of a plate with a purified hCG protein extracted from human urine at a concentration of 10 µg/mL overnight at 4C. The plate was washed 3 times with ELISA Wash Buffer and blocked with 300 ul of StartingBlock (PBS) Blocking Buffer for at least 1 hour at room temperature. After washing 100ul of hCG monoclonal antibody was added to wells in duplicate at 10, 5, 2.5, 1.25, 0.62, 0.31, 0.15, 9.5, 0.078, 0.039, 0.019 and 0 µg/mL concentrations, and the samples were incubated for 2 hours at room temperature. The plate was washed, and then incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature, and washed again with ELISA Wash Buffer. The plate was developed by incubating 100ul per well of 1-Step Ultra TMB substrate per well for 10 minutes at room temperature in the dark. The reaction was stopped with 100ul per well of 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.
hCG / Chorionic Gonadotropin Antibody - Immunohistochemistry analysis of hCG was performed on human placenta tissue and is showing positive staining in extra cellular spaces. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with hCG monoclonal antibody at a dilution of 1:20, overnight at 4C in a humidified chamber without (left panel) or with hCG antibody control (right panel). Tissues were washed, and incubated with secondary antibody (conjugated with HRP) for 30 min at room temperature in a humidified chamber. Detection was performed using DAB substrate kit. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting. Images were taken at 20x magnification.
hCG / Chorionic Gonadotropin Antibody - Immunoprecipitation of hCG proteins was performed by spiking 5 ugs of purified hCG protein extracted from human urine into 1ml of 1:2 diluted human urine sample. Antigen-antibody complexes were formed by incubating with 5ug of hCG antibody overnight on end over end tube rotator at 4C. The immune complexes were captured by using 50ul Protein A/G Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% milk for at least 1 hour at room temperature. hCG was detected at ~32 and 22 kD using hCG monoclonal antibody at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
hCG / Chorionic Gonadotropin Antibody - Direct ELISA analysis of hCG proteins was performed by coating wells of a plate with a purified hCG protein extracted from human urine at a concentration of 10 µg/mL overnight at 4C. The plate was washed 3 times with ELISA Wash Buffer and blocked with 300 ul of StartingBlock (PBS) Blocking Buffer for at least 1 hour at room temperature. After washing 100ul of hCG monoclonal antibody was added to wells in duplicate at 10, 5, 2.5, 1.25, 0.62, 0.31, 0.15, 9.5, 0.078, 0.039, 0.019 and 0 µg/mL concentrations, and the samples were incubated for 2 hours at room temperature. The plate was washed, and then incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature, and washed again with ELISA Wash Buffer. The plate was developed by incubating 100ul per well of 1-Step Ultra TMB substrate per well for 10 minutes at room temperature in the dark. The reaction was stopped with 100ul per well of 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.
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Monoclonal Mouse anti‑Human hCG / Chorionic Gonadotropin Antibody (clone 3002, IHC) LS‑C355424

Monoclonal Mouse anti‑Human hCG / Chorionic Gonadotropin Antibody (clone 3002, IHC) LS‑C355424

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Antibody:
hCG / Chorionic Gonadotropin Mouse anti-Human Monoclonal (3002) Antibody
Application:
IHC, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$857
LS-C355424-1
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
hCG / Chorionic Gonadotropin Mouse anti-Human Monoclonal (3002) Antibody
Application:
IHC, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Chorionic Gonadotropin antibody LS-C355424 is an unconjugated mouse monoclonal antibody to human Chorionic Gonadotropin (hCG). Validated for ELISA, IHC, IP and RIA.
Target
Human hCG / Chorionic Gonadotropin
Synonyms
hCG
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
3002
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Immunogen
Human Chorionic Gonadotropin
Specificity
The antibody targets Chorionic Gonadotropin in ELISA, IP, and RIA applications and shows reactivity with Human samples. It detects Chorionic Gonadotropin which has a predicted molecular weight of approximately 13 kD.
Applications
  • IHC
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.015 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
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