Human HAPLN4

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

NS0-derived rhHAPLN4.

Selected for its ability to recognize human HAPLN4 in the applications listed. In Western Blots, this antibody shows less than 1% cross-reactivity with rhHAPLN1.

• ICC
• Western blot
• Immunoprecipitation

Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human HAPLN4. The detection limit for rhHAPLN4 is approximately 10 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhHAPLN4 from conditioned media of transfected NS0 cells. Immunocytochemistry: This antibody has been used at a concentration of 10 ug/ml to detect HAPLN4 on SHSY5Y human neuroblastoma cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights 557-coupled donkey anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.

Lyophilized

Reconstitute in deionized water

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q86UW8 NCBI: NM_023002 NP_075378.1