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Catalog Number Size Price
LS-C7422-200 200 µl $833 
Antibody

Polyclonal Rabbit anti‑Human H2AFX / H2AX Antibody (aa121‑135, WB) LS‑C7422

Polyclonal Rabbit anti‑Human H2AFX / H2AX Antibody (aa121‑135, WB) LS‑C7422

Antibody:
H2AFX / H2AX Rabbit anti-Human Polyclonal (aa121-135) Antibody
Application:
WB, Func
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$833
LS-C7422-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
H2AFX / H2AX Rabbit anti-Human Polyclonal (aa121-135) Antibody
Application:
WB, Func
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
H2AX antibody LS-C7422 is an unconjugated rabbit polyclonal antibody to H2AX (H2AFX) (aa121-135) from human. It is reactive with human, monkey and rabbit. Validated for Func and WB.
Target
Human H2AFX / H2AX
Synonyms
H2AFX | H2AX | Histone H2A.x | H2A.X | H2A histone family, member X | H2AX histone
Host
Rabbit
Reactivity
Human, Monkey, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
KLH-conjugated, synthetic peptide (C-SATVGPKAPSGGKKA) corresponding to aa121-135 of human histone H2A. X, with an N-Terminus cysteine added for conjugation purposes. The immunizing sequence has 13/15 identical amino acids in mouse. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Rabbit (100%); Gibbon, Rat, Dog, Bovine (93%); Mouse, Hamster (87%); Bat (80%).
Epitope
aa121-135
Specificity
Recognizes human histone H2A. X, Mr 15kD. A band at Mr 100kD was also observed.
Applications
  • Western blot
  • Functional Assay
Usage
Suitable for use in Western Blot and Peptide Inhibition analysis. Western Blot Analysis: A 1:2000-1:10000 dilution of this lot detected histone H2A. X in acid-extracted proteins from HeLa cells. The antibody did not detect recombinant Histone H2A. Peptide Inhibition Analysis: 1 and 10 uM of the immunizing histone H2A. X peptide abolished detection of histone H2A. X by this lot in immunoblot analysis of HeLa acid extracts.
Presentation
0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About H2AFX / H2AX
P16104 NM_002105 NP_002096.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/10/2022