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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GTF2I / TFII I Antibody (clone 3E2) IHC‑plus™ LS‑B12789
Note: This antibody replaces LS-C133226
TFII I antibody LS-B12789 is an unconjugated mouse monoclonal antibody to human TFII I (GTF2I). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B12789-50
50 µg (0.5 mg/ml)
$485

Popular GTF2I / TFII I Products

Anti-GTF2I / TFII I antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Western blot analysis of extracts of 293 cells, using GTF2I antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry
Detection of Human and Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:200 (1 Detection: DAB.
Species: Human, Mouse, Dog, Horse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

GTF2I / TFII I Antibody (clone 3E2) for IHC, IF/Immunofluorescence, WB/Western, ELISA IHC-plus™ LS-B12789

Specifications

Target
Human GTF2I / TFII I
Synonyms
GTF2I, BAP-135, BAP135, BTK-associated protein, 135kD, BTK-associated protein 135, DIWS, IB291, SRF-Phox1-interacting protein, WBSCR6, TFII I, TFII-I, BTKAP1, WBS, GTFII-I, SPIN
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [3E2]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3 - 10 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GTF2I / TFII I antibody was raised against gTF2I (AAH04472, 36 a.a. ~ 274 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human GTF2I
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Tissue lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
About GTF2I / TFII I
Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). P78347 NM_032999 NP_127492.1


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Images

Immunohistochemistry - Paraffin

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Immunofluorescence of monoclonal antibody to GTF2I on HeLa cell. [antibody concentration 10 ug/ml].
Immunofluorescence of monoclonal antibody to GTF2I on HeLa cell. [antibody concentration 10 ug/ml].

Western blot

GTF2I monoclonal antibody (M01), clone 3E2. Western blot of GTF2I expression in human colon.
GTF2I monoclonal antibody (M01), clone 3E2. Western blot of GTF2I expression in human colon.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged GTF2I is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GTF2I is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry - Paraffin

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Immunofluorescence of monoclonal antibody to GTF2I on HeLa cell. [antibody concentration 10 ug/ml].
Immunofluorescence of monoclonal antibody to GTF2I on HeLa cell. [antibody concentration 10 ug/ml].

Western blot

GTF2I monoclonal antibody (M01), clone 3E2. Western blot of GTF2I expression in human colon.
GTF2I monoclonal antibody (M01), clone 3E2. Western blot of GTF2I expression in human colon.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged GTF2I is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GTF2I is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry - Paraffin

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Immunofluorescence of monoclonal antibody to GTF2I on HeLa cell. [antibody concentration 10 ug/ml].
Immunofluorescence of monoclonal antibody to GTF2I on HeLa cell. [antibody concentration 10 ug/ml].

Western blot

GTF2I monoclonal antibody (M01), clone 3E2. Western blot of GTF2I expression in human colon.
GTF2I monoclonal antibody (M01), clone 3E2. Western blot of GTF2I expression in human colon.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged GTF2I is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GTF2I is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry - Paraffin

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Immunofluorescence of monoclonal antibody to GTF2I on HeLa cell. [antibody concentration 10 ug/ml].
Immunofluorescence of monoclonal antibody to GTF2I on HeLa cell. [antibody concentration 10 ug/ml].

Western blot

GTF2I monoclonal antibody (M01), clone 3E2. Western blot of GTF2I expression in human colon.
GTF2I monoclonal antibody (M01), clone 3E2. Western blot of GTF2I expression in human colon.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged GTF2I is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GTF2I is approximately 0.03 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 10/17/2018

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