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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GTF2I / TFII I Antibody (C‑Terminus) LS‑C286383
TFII I antibody LS-C286383 is an unconjugated rabbit polyclonal antibody to TFII I (GTF2I) from human, mouse and rat. Validated for IF and IHC.
Catalog
Size
Price
LS-C286383-100
100 µl
$415

Popular GTF2I / TFII I Products

Anti-GTF2I / TFII I antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Western blot analysis of extracts of 293 cells, using GTF2I antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry
Detection of Human and Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:200 (1 Detection: DAB.
Species: Human, Mouse, Dog, Horse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

GTF2I / TFII I Antibody (C-Terminus) for IHC, IF/Immunofluorescence LS-C286383

Specifications

Target
Human GTF2I / TFII I
Synonyms
GTF2I, BAP-135, BAP135, BTK-associated protein, 135kD, BTK-associated protein 135, DIWS, IB291, SRF-Phox1-interacting protein, WBSCR6, TFII I, TFII-I, BTKAP1, WBS, GTFII-I, SPIN
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:500)
  • Immunofluorescence (1:50 - 1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
C-Terminus
Specificity
Region between residues 950 and the C-terminus (residue 998) of human general transcription factor II-I using the numbering given in entry NP_127492.1 (GeneID 2969).
Usage
Immunohistochemistry: Antigen retrieval is recommended. Antigen retrieval with citrate buffer will enhance staining. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated. Human controls: B-cell LympH oma, Breast Carcinoma, Colon Carcinoma, Laryngeal Squamous Cell Carcinoma, Linitis Plastica Stomach Cancer, Metastatic LympH Node, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Skin Basal Cell Carcinoma, Skin Squamous Cell Carcinoma, Small Cell Lung Cancer, Stomach Adenocarcinoma, Testicular Seminoma. Mouse controls: Squamous Cell Carcinoma.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
TBS, 0.09% Sodium Azide, 0.1% BSA
Storage
Store at 2°C to 8°C for up to 1 year.
Restrictions
For research use only.
About GTF2I / TFII I
Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). P78347 NM_032999 NP_127492.1


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Images

Immunohistochemistry

Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human prostate adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.
Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human prostate adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.

Immunohistochemistry

Detection of Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.
Detection of Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.

Immunohistochemistry

Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human breast adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100.
Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human breast adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100.

Immunofluorescence

Detection of Human GTF2I/TFII-I by Immunofluorescence. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100. Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG (Invitrogen) used at a dilution of 1:500.
Detection of Human GTF2I/TFII-I by Immunofluorescence. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100. Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG (Invitrogen) used at a dilution of 1:500.

Immunohistochemistry

Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human prostate adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.
Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human prostate adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.

Immunohistochemistry

Detection of Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.
Detection of Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.

Immunohistochemistry

Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human breast adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100.
Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human breast adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100.

Immunofluorescence

Detection of Human GTF2I/TFII-I by Immunofluorescence. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100. Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG (Invitrogen) used at a dilution of 1:500.
Detection of Human GTF2I/TFII-I by Immunofluorescence. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100. Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG (Invitrogen) used at a dilution of 1:500.

Immunohistochemistry

Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human prostate adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.
Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human prostate adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.

Immunohistochemistry

Detection of Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.
Detection of Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.

Immunohistochemistry

Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human breast adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100.
Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human breast adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100.

Immunofluorescence

Detection of Human GTF2I/TFII-I by Immunofluorescence. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100. Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG (Invitrogen) used at a dilution of 1:500.
Detection of Human GTF2I/TFII-I by Immunofluorescence. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100. Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG (Invitrogen) used at a dilution of 1:500.

Immunohistochemistry

Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human prostate adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.
Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human prostate adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.

Immunohistochemistry

Detection of Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.
Detection of Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:250.

Immunohistochemistry

Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human breast adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100.
Detection of Human GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human breast adenocarcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100.

Immunofluorescence

Detection of Human GTF2I/TFII-I by Immunofluorescence. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100. Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG (Invitrogen) used at a dilution of 1:500.
Detection of Human GTF2I/TFII-I by Immunofluorescence. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:100. Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG (Invitrogen) used at a dilution of 1:500.

Requested From: United States
Date Requested: 10/16/2018

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